Image: a, Venn diagrams showing genes upregulated via RNA-seq experiments in four cell types comprising components of the TME (PANC-1, MIA PaCa-2, CAF and CD8+ T cells) and inhibited in two cell types (PANC-1 and MIA PaCa-2). A total of 15 genes were upregulated by AMP and inhibited by quemliclustat. Differentially expressed genes are defined as an increase or decrease of 50% log2 fold change with FDR ≤ 0.05. b, Heatmap of log2 fold change of RT−PCR values for NR4A family genes treated with adenosine-generating conditions or adenosine 2a receptor/adenosine 2b receptor agonist conditions and their corresponding inhibitors in expanded cell types in the TME. c, NR4A family genes can be upregulated to varying degrees in cell types comparing human PDAC in response to chemotherapy.
Our CAFs have a long history of being utilized to study the TME. A growing trend we're seeing is the use of these cells in 3D models. Our colorectal, pancreatic, breast, and ovarian CAFs have all been used in 3D TME models in recent publications.
We have a large selection of CAFs, including around a dozen types of normal CAFs and several hTERT immortalized CAFs. Check them all out below.