Neuromics loves to highlight when our reagents are used in meaningful research. Recently, two of our customers published studies using our Tuj-1 mouse monoclonal antibody (MO15013) in their neuroscience research.

The first study looks at various fibrillars that could be used to work with Schwann cells (SCs) and dorsal root ganglia (DRG) in tissue engineering applications. They aim to assess the differences in biological behavior of the SCs and DRG when interacting with electrospun nanofibers and extruded microfibres. With our Tuj-1 antibody, they find that the extruded microfibers produce higher motility and cell-cell interaction, while a higher cell-material interaction with a lower cell motility is observed for the electrospun nanofibers. As a result, the authors conclude that there is more potential for axonal growth with the extruded microfibers when compared to the electrospun nanofibers.

Image: Tuj-1 immunofluorescence images of DRG after 3 days of culture on the different substrates.

Read more to see the second publication

The second publication explores Glucose Synthase Kinase-3 (GSK-3) inhibition, and how it could lead to neurogenesis as a potential treatment for spinal cord injuries. When culturing various types and species of multipotent stem cells in vitro and inhibiting GSK-3, they found an increased number of neurons. Our Tuj-1 (MO15013) mouse monoclonal antibody was used to help recognize this increase in neurons around the site of injury. In the future, the combination of treating animals with ependymal stem/progenitor cells, human embryonic stem cells–derived neural progenitors, and a GSK-3 inhibitor could be a viable treatment for spinal cord injuries with further supporting research.

Image: GSK-3 inhibition increases the expression of the Tuj-1 neurogenesis marker. Expression of β-catenin, as a control for GSK-3 inhibition, and the neuronal marker Tuj-1 in epSPCs compared to control cells.

 

Citations:

  1. Fernando Gisbert Roca, Jorge Mas Estelles, Manuel Monleon Pradas, and Cristina Martinez-Ramos. (2020). Axonal Extension from Dorsal Root Ganglia on Fibrillar and Highly Aligned poly(lactic acid)-polypyrrole Substrates Obtained by Two Different Techniques: Electrospun Nanofibres and Extruded Microfibres.International Journal of Biological Macromolecules, 163, 1959-1969. doi: 10.1016/j.ijbiomac.2020.09.181
  2. Francisco Javier Rodriguez-Jimenez, Angel Vilches, Maria Amparo Perez-Arago, Eleonora Clemente, Raquel Roman, Juliette Leal, Ana Artero Castro, Santos Fustero, Victoria Moreno-Manzano, Pavla Jendelova, Miodrag Stojkovic, and Slaven Erceg. (2020). Activation of Neurogenesis in Multipotent Stem Cells Cultured In Vitro and in the Spinal Cord Tissue After Severe Injury by Inhibition of Glycogen Synthase Kinase-3.Neurotherapeutics. doi: 10.1007/s13311-020-00928-0