Product Details
Catalog Number: PC35101
Applications: Cell Assays
Type: Primary Cells
Species Reactivity: Rat
Downloads: Datasheet (pdf)
Product Sizes
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1 Hippocampal pair$215.00Add to Cart

Primary Rat Hippocampal Cells are live neurons isolated from micro surgically dissected regions of day 18 embryonic Sprague/Dawley rat brain. These cells are prepared fresh each week and shipped in a nutrient rich medium that keeps the cells alive for up to 7 days under refrigeration.

For long term culture protocol (120 days) see: Todd GK, Boosalis CA, Burzycki AA, Steinman MQ, Hester LD, Shuster PW, et al. (2013) Towards Neuronal Organoids: A Method for Long-Term Culturing of High-Density Hippocampal Neurons. PLoS ONE 8(4): e58996. https://doi.org/10.1371/journal.pone.0058996

Images

(A) F-Aβ40 uptake; (B) Uptake of AF633-Trf; (C) Superimposition of images A and B; (D) Overlay of fluorescence images on the DIC image of RPH neurons. (E) Uptake of F-Aβ40; (F) No significant neuronal uptake of AF633-Trf at 4°C; (G) Superimposition of images D and E on the DIC image of RPH neurons; (H) Uptake of F-Aβ40; (I) No significant cellular uptake of AF633-Trf was observed; (J) Superimposition of images H & I on the DIC image of neurons.

(A) F-Aβ40 uptake; (B) Uptake of AF633-Trf; (C) Superimposition of images A and B; (D) Overlay of fluorescence images on the DIC image of RPH neurons. (E) Uptake of F-Aβ40; (F) No significant neuronal uptake of AF633-Trf at 4°C; (G) Superimposition of images D and E on the DIC image of RPH neurons; (H) Uptake of F-Aβ40; (I) No significant cellular uptake of AF633-Trf was observed; (J) Superimposition of images H & I on the DIC image of neurons.

Hippocampal neurons stained with Tau and Doublecortin

E18 hippocampal neurons stained with Tau (red, CH22113) and Doublecortin (green, MO22113). The two proteins overlap in the proximal dendrites (yellow) Axons (low doublecortin content) are red. Blue staining is the nuclear DNA

Hippocampal neurons stained with Tau and Doublecortin

E18 hippocampal neurons stained with Tau (red, CH22113) and Doublecortin (green, MO22113). The two proteins overlap in the proximal dendrites (yellow) Axons (low doublecortin content) are red. Blue staining is the nuclear DNA

Embryonic day-18 primary rat hippocampal cells (PC 35101) grown over pLL-coated 6-well plate for 48 hours. Green: anti-rat TAU/MAPT (CH22113), blue: DAPI. Scale bar = 25 um.

Embryonic day-18 primary rat hippocampal cells (PC 35101) grown over pLL-coated 6-well plate for 48 hours. Green: anti-rat TAU/MAPT (CH22113), blue: DAPI. Scale bar = 25 um.

Embryonic day-18 primary rat hippocampal cells (PC 35101) grown over pLL-coated 6-well plate for 48 hours. Green: anti-rat TAU/MAPT (CH22113), blue: DAPI. Scale bar = 25 um.

Embryonic day-18 primary rat hippocampal cells (PC 35101) grown over pLL-coated 6-well plate for 48 hours. Green: anti-rat TAU/MAPT (CH22113), blue: DAPI. Scale bar = 25 um.