Product Details
Catalog Number: PC35102
Applications: Cell Assays
Type: Primary Cells
Storage: Store for up at 7 days at 4°C-8°C.
Shipping: Refrigerated (Polar Packs)
Species Reactivity: Rat
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
1 Cortical Pair$215.00Add to Cart

Primary Rat Cortical Cells are live neurons isolated from micro-surgically dissected regions of day 18 embryonic Sprague/Dawley rat brain. These cells are prepared fresh each week and shipped in a nutrient rich medium that keeps the cells alive for up to 14 days under refrigeration.

Please note we also provide Adult Neurons in Qtys of 4 (or more) for $995.  To order call brett @ 866-350-1500 or e-mail: brett@neuromics.com.

Images

Neurite outgrowth of rat E18 cortical neurons in a 96-well microplate. Left: 20x HD phase image of primary neurons, 96 hours post plating ; Middle: Image segmentation of neurites(yellow) and cell body cluster (raspberry); Right: Concentration and time dependent inhibition of neurite outgrowth with the protein kinase C inhibitor, Ro-31-8220 (mean ± SD; n=6 per condition. Courtesy of Lauren McGillicuddy, Essen Biosciences.

Neurite outgrowth of rat E18 cortical neurons in a 96-well microplate. Left: 20x HD phase image of primary neurons, 96 hours post plating ; Middle: Image segmentation of neurites(yellow) and cell body cluster (raspberry); Right: Concentration and time dependent inhibition of neurite outgrowth with the protein kinase C inhibitor, Ro-31-8220 (mean ± SD; n=6 per condition. Courtesy of Lauren McGillicuddy, Essen Biosciences.

Pak1 colocalizes with muhtt aggregates. (A) Muhtt548-GFP and Pak1 were transfected into rat E18 striatal neurons. (B) Muhtt548-GFP was transfected into rat E18 cortical neurons.(C) HD brain (Grade III) slices were stained with anti-htt (EM48) and anti-Pak1, anti-htt (EM48) only or anti-Pak1 only or no primary antibodies using PicTure-Double staining kit.(D) HD brain (Grade III) and control brain samples were stained with anti-Pak1 antibody using Vectastain Avidin kit.

Pak1 colocalizes with muhtt aggregates. (A) Muhtt548-GFP and Pak1 were transfected into rat E18 striatal neurons. (B) Muhtt548-GFP was transfected into rat E18 cortical neurons.(C) HD brain (Grade III) slices were stained with anti-htt (EM48) and anti-Pak1, anti-htt (EM48) only or anti-Pak1 only or no primary antibodies using PicTure-Double staining kit.(D) HD brain (Grade III) and control brain samples were stained with anti-Pak1 antibody using Vectastain Avidin kit.