Product Details
Catalog Number: PR22000
Type: Peptide
Storage: Store at 2°C-8°C
Shipping: Ambient
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
100 ug Blocking Protein$295.00Add to Cart
50 ug$395.00Add to Cart

Human GFAP is a 49,749 dalton protein (432 amino acids) expressed in astrocytes. GFAP is an intermediate filament protein and acts as an intracellular structural component of the cytoskeleton. During embryonic and fetal life, GFAP is also expressed by radial glial cells of the CNS. Various mutations of the GFAP gene in humans produce Alexander's disease, one of the leukodystrophies.

Images

Mixed neuron-glial cultures stained with Mouse Monoclonal GFAP, and Chicken Polyclonal Neurofilament-NF-L (green). The GFAP antibody stains the network of astrocytes in these cultures, while the NF-L antibody stains neurons and their processes. The blue channel shows the localization of DNA. This antibody also works on formalin fixed paraffin embedded brain tissues. Protocol on Datasheet.

Mixed neuron-glial cultures stained with Mouse Monoclonal GFAP, and Chicken Polyclonal Neurofilament-NF-L (green). The GFAP antibody stains the network of astrocytes in these cultures, while the NF-L antibody stains neurons and their processes. The blue channel shows the localization of DNA. This antibody also works on formalin fixed paraffin embedded brain tissues. Protocol on Datasheet.

Image of GFAP (red) staining of astrocytes in primary culture (DAPI, blue).

Image of GFAP (red) staining of astrocytes in primary culture (DAPI, blue).

Western blot of whole rat cerebellum homogenate stained with CPCA-GFAP, at dilution of 1:100,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~50kDa corresponds to rodent GFAP. A lower band at ~45kDa is derived from the GFAP molecule.

Western blot of whole rat cerebellum homogenate stained with CPCA-GFAP, at dilution of 1:100,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~50kDa corresponds to rodent GFAP. A lower band at ~45kDa is derived from the GFAP molecule.

Strip blot of rat spinal cord protein extract stained with GFAP. A prominent band at about 55 kDa corresponds to the major isoform of GFAP.

Strip blot of rat spinal cord protein extract stained with GFAP. A prominent band at about 55 kDa corresponds to the major isoform of GFAP.