Membrane-Based Antibody Arrays (C-Series) are tools for screening and comparing expression levels of many cytokines, growth factors, proteases, soluble receptors, and other proteins in a wide variety of sample types.

The C-Series Arrays utilize the sandwich immunoassay principle, wherein a panel of capture antibodies is printed on a nitrocellulose membrane solid support (usualy 2.5 cm x 3 cm). The array membranes are processed similarly to a Western blot (chemiluminescent readout). Signals are then visualized on x-ray film or a digital image, allowing densitometry data collection and calculation of fold-changes for each detected protein. The entire procedure can be completed in 1 day, and is simple enough to permit even the novice researcher to successfully collect data with very few pitfalls and little or no optimization.

Many biological processes such as apoptosis, inflammation, angiogenesis, immune response and migration often accompany changes of cytokine expression levels. Because of the extensive cross-talk between cytokines, a complete analysis of biological responses and functions must be obtained through multiplex assays. The C-Series Antibody Arrays allow a much broader view of protein activity than can be obtained with single-target ELISAs and Western blots. Moreover, antibody array screening improves the chances for discovering key factors, disease mechanisms or biomarkers related to cytokine signaling.

Identification of cytokines in VF conditioned medium. A cytokine antibodies array (RayBiotech, Norcross, GA, Cat. No.AAM-CYT-3-4) identifies the presence of chemoattractant and inflammation peptides including RANTES, eotaxin, eotaxin-2, IL-4, and IL-1α. Experiments were performed according to manufacturers’ protocols. Supporting information. Coherent Anti-Stokes Raman Scattering Imaging of Lipids inCancer Metastasis. Dr. Ji-Xin Cheng et al. Purdue Cancer Center.