HGMECs were isolated from normal human kidney glomeruli. Passage 2, cells are ship in proliferating culture with a confluency of >90 %. ENDO-Growth medium containing 5% serum and growth supplements are recommended for culture. Cells have an average additional population doubling levels >20 when cultured.
Characterization of the cells
Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence
Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
Cytoplasmic PECAM1 >95% positive by immunofluorescence
HGMECs are negative for HIV-1, HBV, HCV, and mycoplasma.