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Type: Primary Cells
Applications: Cell Assays
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
HPMECs initiated by elutriation from dissociated normal human Prosate tissue. Passage 3 cells are ship in proliferating culture with a confluency of >90 %. ENDO-Growth medium containing 5% serum and growth supplements are recommended for culture. Cells have an average additional population doubling levels >20 when cultured.
Characterization of the cells
Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence
Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
Cytoplasmic PECAM1 >95% positive by immunofluorescence
HPMECs are negative for HIV-1, HBV, HCV, and mycoplasma.