Catalog NumberSizePrice (USD)Shopping Cart
HEC15500,000+ Cells Frozen$789.00Buy Now | Add to Cart
HEC15-T25500,000+ Cells in T25 Flask$789.00Buy Now | Add to Cart
 
Type: Primary Cells
 
Applications: Cell Assays
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Description/Data:

HSIMECs initiated by elutriation from dissociated normal small intestine tonsil tissue. Passage 3 cells are ship in proliferating culture with a confluency of >90 %. ENDO-Growth medium containing 5% serum and growth supplements are recommended for culture. Cells have an average additional population doubling levels >16 when cultured.

Characterization of the cells
  • Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence
  • Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
  • Cytoplasmic PECAM1 >95% positive by immunofluorescence

HSIMECs are negative for HIV-1, HBV, HCV, and mycoplasma.

**cells are shipped at 90% confluence. However, confluence can be lost during shipping. Please allow for 1-4 days to reach 90% confluence again.