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Type: Goat IgG
 
Applications: IHC; WB; E
ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; WB=Western blotting; FC=Flow Cytometry; IP=Immunoprecipitation; E=ELISA; NB=Neutralization of Bioactivity; FACS; All Fluorecent Images;
Species Reactivity: M; R
B=Bovine; Ca=Cat; Ch=Chicken; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Ze=Zebrafish; na
Format: Affinity Purified - liquid
 
Immunogen: Purified, E. coli-derived, recombinant rat Nestin (rrNestin; aa 544 - 776).
 
Description/Data:

Mouse nestin is a 206,994 dalton protein (1864 amino acids) expressed in neural crest cells, CNS neural stems cells, as well as a few non-neural cell types in the embryo, including cells within the pancreatic islets of Langerhans and the limb bud. Nestin is an intermediate filament-associated protein, and contributes to the cytoskeleton.

Image: Neural progenitors were labeled with anti-rat Nestin polyclonal antibody (Cat#:GT15114) and stained with conjugated donkey anti-goat secondary anti-body (green). Differentiated neurons were labeled with neuron-specific mouse anti-β-III tubulin/ Tuj1-(Cat#MO15013) monoclonal antibody (red). Nuclei were stained with DAPI (blue).

Other Reagents to Consider:

Nestin-Mouse Monoclonal

Nestin-Mouse Monclonal

Nestin Chicken

Tuj 1 (Neuron-specific class III beta-tubulin)-Mouse Monclonal

Tuj 1 (Neuron-specific class III beta-tubulin)-Chicken Polyclonal

All Neuron-Glial Markers

Stem Cell Reagents