Trust Your Results

We strive to provide protocols that are easy to understand so you can produce results you can trust. Here's an example protocol for making long term cultures using our E18 and E20 Rat Primary Neuronal Tissue.

Method for Long-Term Culturing of High-Density Hippocampal Neurons

Figure: Protocol to Prepare Hippocampal Neuronal Cultures.Diagrammatic representation of the protocol used for preparing 120 DIV E18 hippocampal cultures. Citation: Todd GK, Boosalis CA, Burzycki AA, Steinman MQ, Hester LD, Shuster PW, et al. (2013) Towards Neuronal Organoids: A Method for Long-Term Culturing of High-Density Hippocampal Neurons. PLoS ONE 8(4): e58996. doi:10.1371/journal.pone.0058996