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Type: Sheep IgG
 
Applications: ICC; WB
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H; M
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Affinity Purified - liquid
 
Immunogen: E. coli derived recombinant human ZIC3. His380-Val467 Accession # O60481.
 
Description/Data:
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ZIC3 or zinc finger protein of the cerebellum 3 is a 50 kDa (predicted) member of the GLI C2H2type Zn finger protein family. It is involved in the maintenance of pluripotency in ES cells by preventing endodermal lineage specification in embryonic stem cells. The protein is also expressed in the cerebellum.

ZIC3 staining of immersion fixed A172 human glioblastoma cell line using Human/Mouse ZIC3 Antigen Affinity-purified Polyclonal Antibody at 10 µg/mL for 3 hours at room temperature. Cells were stained using the a 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel ) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. Protocol on data-sheet.

Defects in ZIC3 are the cause of visceral heterotaxy X-linked type 1 (HTX1). A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects.

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Images: Western blot shows lysates of 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse ZIC3 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for ZIC3 at approximately 56 kDa (as indicated). Protocol on data-sheet.