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| Catalog Number | Size | Price (USD) | Shopping Cart |
|---|---|---|---|
| RA19071 | 100 ul | $325.00 | Buy Now | Add to Cart |
Influenzavirus A is a genus of the Orthomyxoviridae family of viruses and is a negative sense, single-stranded, segmented RNA virus. Based on the antigenicity of the glycoproteins, influenza A viruses are subdivided into sixteen H (H1-H16) and nine N (N1-N9) subtypes. A standard nomenclature for influenza virus isolates lists the influenza virus type (A or B), the host species (omitted if human origin), the geographical site, year of isolation, and the HA and NA subtype, for example: A/California/14/2009 (H1N1).
The main antigenic determinants of influenza A and B viruses are the hemagglutinin (HA) and neuraminidase (NA) transmembrane glycoproteins. Projections of HA and NA cover the surface of the virus particle. NA forms a tetramer with an average molecular weight of 220 kDa (~55 kDa per monomer). The matrix (M) protein of influenza A virus is one of the two group-specific internal proteins of the virion, The non-structural protein (NP) exists as a homeodimer (molecular weight of 52 kDa) consisting of two identical monomers (each ~26 kDa).
Image: Western blot analysis of Influenza A Virus (subtype H1N1). 3 ug of intact A/California/14/2009 H1N1 virions were loaded on SDS-PAGE, transferred to a PVDF membrane and detected with rabbit anti-Influenza A Virus (subtype H1N1) Polyclonal Antibody at a 1:1,000 dilution. Goat anti-rabbit secondary antibody was used at 1:10,000 dilution. The antibody detects: Hemagglutinin (HA) ~75 kDa, Neuraminidase monomer (NA) ~55 kDa, Matrix (M) ~26 kDa and Non-structural Protein monomer (NP) ~26-27 kDa (M and NP often co-migrate as 1 band).