GFAP |
Featured Reagents:
Other Reagents to Consider:
Neuronal-Glial Markers -Astrocytes, Glia, Microglia, Olidogodendrocytes, Progenitors and Schwann Cell Markers
Neurotrophins and Growth Factor Antibodies
David H. McDougal,Gerlinda E. Hermann, and Richard C. Rogers. Vagal Afferent Stimulation Activates Astrocytes in the Nucleus of the Solitary Tract Via AMPA Receptors: Evidence of an Atypical Neural–Glial Interaction in the Brainstem. The Journal of Neuroscience, 28 September 2011, 31(39): 14037-14045; doi: 10.1523/JNEUROSCI.2855-11.2011.
...GFAP Chicken Neuromics CH22102 1:500...
Sue H. Huh, Young C. Chung, Ying Piao, Min Y. Jin, Hyo J. Son, Nam S. Yoon, Joo Y. Hong, Youngmi K., Pak, Yoon S. Kim, Jong K. Hong, Onyou Hwang#and Byung K. Jin. Ethyl Pyruvate Rescues Nigrostriatal Dopaminergic Neurons by Regulating Glial Activation in a Mouse Model of Parkinson’s Disease. Published online before print June 17, 2011, doi: 10.4049/The Journal of Immunology.
...GFAP, 1:5000;Neuromics)...
A. J. Mercer, K. Rabl, G. E. Riccardi, N. C. Brecha, S. L. Stella, Jr, and W. B. Thoreson Location of Release Sites and Calcium-Activated Chloride Channels Relative to Calcium Channels at the Photoreceptor Ribbon Synapse. J Neurophysiol, Jan 2011; 105: 321 - 335.
...antibody against glial fibillary acidic protein (GFAP) was used at a dilution of 1:1,000 (catalog no. CH 22102; Neuromics, Edina, MN) to identify Muller cells in the salamander retina...
Jiaorong Chen, Nobuo Terada, Nobuhiko Ohno, Sei Saitoh, Yurika Saitoh and Shinichi Ohno. Immunolocalization of membrane skeletal protein, 4.1G, in enteric glial cells in the mouse large intestine. Neuroscience Letters. Volume 488, Issue 2, 20 January 2011, Pages 193-198
...chicken polyclonal anti-glial fibrillary acidic protein (GFAP) Ab (Neuromics Antibodies, Minneapolis, MN, USA)...
Mariano Guardia Clausi, Laura A Pasquini, Eduardo F Soto and Juana M Pasquini. (2010) Apotransferrin-induced recovery after hypoxic/ischaemic injury on myelination. ASN NEURO 2(5):art:e00048.doi:10.1042/AN20100020
...Anti-GFAP (where GFAP is glial fibrillary acidic protein) and anti-Nestin antibodies were purchased from Neuromics (Edina, MN, U.S.A.)...
Young C. Chung, Sang R. Kim and Byung K. Jin. Paroxetine Prevents Loss of Nigrostriatal Dopaminergic Neurons by Inhibiting Brain Inflammation and Oxidative Stress in an Experimental Model of Parkinson’s Disease. Published online before print June 21, 2010, doi: 10.4049/jimmunol.1000208.
... glial fibrillary acidic protein (GFAP; 1:5000; Neuromics, Edina)...
Verónica I. Landoni, Marcelo de Campos-Nebel, Pablo Schierloh, Cecilia Calatayud, Gabriela C. Fernandez, M. Victoria Ramos, Bárbara Rearte, Marina S. Palermo, and Martín A. Isturiz. Shiga Toxin 1-Induced Inflammatory Response in Lipopolysaccharide-Sensitized Astrocytes Is Mediated by Endogenous Tumor Necrosis Factor Alpha. Infection and Immunity, March 2010, p. 1193-1201, Vol. 78, No. 3. doi:10.1128/IAI.00932-09
...incubated for 2 h with IgY chicken anti-GFAP(1:300 dilution; Neuromics, Edina, MN). After washing, appropriate secondary FITC-conjugated goat anti-chicken IgY antibody (1:250 dilution; Neuromics, Edina, MN) was added and incubated for 1 h. Immunostaining...
Min-Tsai Liu, Yung-Hui Kuan, Jingwen Wang, René Hen, and Michael D. Gershon. 5-HT4 Receptor-Mediated Neuroprotection and Neurogenesis in the Enteric Nervous System of Adult Mice. The Journal of Neuroscience, August 5, 2009, 29(31):9683-9699; doi:10.1523/JNEUROSCI.1145-09.2009.
Antibodies Used:
- GFAP (Chicken-Cat#: CH22102)-Dilution 1:2000
- Musashi-1 (Rabbit-Cat#: RA14128)-Dilution 1:100 (IHC)/1:1000(WB)
- Neurofilament H (Chicken-Cat#: CH22104)-Dilution 1:2000
- S100B (Rabbit Polyclonal-Cat#: RA25022)-Dilution 1:1000
V. Milleta, C.P. Moiola, J.M. Pasquini, E.F. Soto and L.A. Pasquini. Partial inhibition of proteasome activity enhances remyelination after cuprizone-induced demyelination. Experimental Neurology Volume 217, Issue 2, June 2009, Pages 282-296.
...anti-GFAP were from Neuromics...
Ulla C. Kopp, Olaf Grisk, Michael Z Cicha, Lori A Smith, Antje Steinbach, Torsten Schluter, Nicole Mahler, and Tomas Hokfelt Dietary sodium modulates the interaction between efferent renal sympathetic nerve activity and afferent renal nerve activity: role of endothelin. Am J Physiol Regulatory Integrative Comp Physiol, May 2009; doi:10.1152/ajpregu.91029.2008
...hrs at 4oC. ETB-R. The sections were incubated with antisera against glial fibrillary acidic protein (GFAP, mouse;1:1,000, Neuromics, Edina, MN), a marker for satellite cells in DRG and unmyelinated ensheathing Schwann cells in the peripheral nervous system...
Sun Jin-qiao, Sha Bin, Zhou Wen-hao and Yang Yi. Basic fibroblast growth factor stimulates the proliferation and differentiation of neural stem cells in neonatal rats after ischemic brain injury. doi:10.1016/j.braindev.2008.06.005.
For the immunofluorescence assays, sections from the SVZ were washed (0.1 M Tris, pH 7.6, 15 min), denatured (2 N HCl, 37oC, 30 min), rinsed (0.1 M P10 min), incubated with 1% H2O2 in 0.1 M Tris for 30 min, rinsed, blocked (10% normal goat serum, 37oC, 30 min).
...GFAP (1:100, Neuromics)...
Aaron Hirko , Renee Dallasen , Sachiko Jomura , Yan Xu. Modulation of Inflammatory Responses after Global Ischemia by Transplanted Umbilical-Cord Matrix Stem Cells. First published online August 21, 2008; doi:10.1634/stemcells.2008-0075
...The primary antibodies were incubated overnight. at 4°C using mouse anti-GFAP (1 :200) from. Neuromics (Edina, MN)...
Jan Mulder, Tibor Harkany, Katalin Czollner, Thomas I.F.H. Cremers, Jan N. Keijser, Csaba Nyakas, Paul G.M. Luiten. Galantamine-induced behavioral recovery after sublethal excitotoxic lesions to the rat medial septum. doi:10.1016/j.bbr.2005.04.019
..mouse anti-GFAP (1:800; Neuromics, Edina, MN)...
David J. Gifondorwa, Mac B. Robinson, Crystal D. Hayes, Anna R. Taylor, David M. Prevette, Ronald W. Oppenheim, James Caress, and Carolanne E. Milligan. Exogenous Delivery of Heat Shock Protein 70 Increases Lifespan in a Mouse Model of Amyotrophic Lateral Sclerosis. doi:10.1523/JNEUROSCI.4057-07.2007.
...The primary antibodies used for glial cell activation were glial fibrillary acidic protein (GFAP; RA22101 ,Neuromics, Minneapolis, MN)...








