Paul M. Georgea, Tonya M. Blissb, Thuy Huab, Alex Leed, Byeongtaek Oh, Alexa Levinson, Swapnil Mehta, Guohua Sun, Gary K. Steinberg. Electrical preconditioning of stem cells with a conductive polymer scaffold enhances stroke recovery. doi.org/10.1016/j.biomaterials.2017.07.020.

...anti βIII-tubulin (1:500, Neuromics, Edina, MN)...

Wenqing Yan, Wenwen Liu, Jieyu Qi, Qiaojun Fang, Zhaomin Fan, Gaoying Sun, Yuechen Han, Daogong Zhang, Lei Xu, Mingming Wang, Jianfeng Li, Fangyi Chen, Dong Liu, Renjie Chai, Haibo Wang. A Three-Dimensional Culture System with Matrigel Promotes Purified Spiral Ganglion Neuron Survival and Function In Vitro. Mol Neurobiol (2017). doi:10.1007/s12035-017-0471-0 

... 100 in PBS and immersed in blocking solution (0.1% Triton X-100, 8% don- key serum, 1% bovine serum albumin, and 0.02% sodium azide in PBS) at room temperature for 1 h. The samples were incubated with primary antibodies against Tuj1 (1:1000, Neuromics, USA), NeuN ... 

Xianmin Wu,  Xiaofei Li, Yongdong Song, He Li, Xiaohui Bai, Wenwen Liu, Yuechen Han, Lei Xu, Jianfeng Li, Daogong Zhang, Haibo Wang, & Zhaomin Fan. Allicin protects auditory hair cells and spiral ganglion neurons from cisplatin - Induced apoptosis. Neuropharmacology. Available online 3 January 2017. http://dx.doi.org/10.1016/j.neuropharm.2017.01.001

... Antibody to Tuj 1 was acquired from Neuromics (Edina, USA). ... The primary antibodies were cleaved-caspase-3 (1:400), cleaved-caspase-9 (1:400), cytochrome c (1: 50), P53 (1: 50), Tuj-1 (1: 1000, Neuromics) and Sox-2 (1:50, Santa Cruz, CA)... 

Di Jiang, Jintao Du, Xuemei Zhang, Wei Zhou, Lin Zong, Chang Dong, Kaitian Chen, Yu Chen, Xihui Chen, and Hongyan Jiang. miR-124 promotes the neuronal differentiation of mouse inner ear neural stem cells. International Journal of Molecular Medicine. November 2016. Volume 38 Issue 5 Print ISSN: 1107-3756 Online ISSN:1791-244X. DOI: 10.3892/ijmm.2016.2751

... Undifferentiated and differ- entiated inner ear NSCs were fixed with 4% paraformaldehyde and immunostained with antibodies specific for BrdU (C2181; 1:10,000; Sigma), Tuj1 (class III β-tubulin; MO15013; 1:250; Neuromics, Edina, MN, USA), p27Kip1 (554069; 1:150; BD ...

Laura A. Volpicelli-Daley, Hisham Abdelmotilib, Zhiyong Liu, Lindsay Stoyka, João Paulo Lima Daher, Austen J. Milnerwood, Vivek K. Unni, Warren D. Hirst, Zhenyu Yue, Hien T. Zhao, Kyle Fraser, Richard E. Kennedy, and Andrew B. West. G2019S-LRRK2 Expression Augments α-Synuclein Sequestration into Inclusions in Neurons. The Journal of Neuroscience, 13 July 2016, 36(28): 7415-7427; doi: 10.1523/JNEUROSCI.3642-15.2016

... Primary antibodies were diluted in blocking buffer. Primary antibodies included the following: synuclein mouse antibody (Syn1, BD Biosciences), LRRK2 (c41-2; Abcam), pS1292-LRRK2(MJFR-19-7-8, Abcam), VDAC (NeuroMab), and Tuj1 (Neuromics Antibodies). ...

Vitor Y. R. Soares, Nadia A. Atai, Takeshi Fujita, Sonam Dilwali, Sarada Sivaraman, Lukas D. Landegger, Fred H. Hochberg, Carlos A. P. C. Oliveira, Fayez Bahmad, Xandra O. Breakefield and Konstantina M. Stankovic. Extracellular vesicles derived from human vestibular schwannomas associated with poor hearing damage cochlear cells. Neuro Oncol (2016) doi: 10.1093/neuonc/now099 First published online: May 18, 2016

...chicken anti-ObRB (1:5000; CH14104, Neuromics; Edina, MN),chicken anti-ObRB (1:5000; CH14104, Neuromics; Edina, MN)...

Thomas V. Johnson; Ericka N. Oglesby; Matthew R. Steinhart; Elizabeth Cone-Kimball; Joan Jefferys; Harry A. Quigley. Time-Lapse Retinal Ganglion Cell Dendritic Field Degeneration Imaged in Organotypic Retinal Explant Culture. Investigative Ophthalmology & Visual Science January 2016, Vol.57, 253-264. doi:10.1167/iovs.15-17769

...Retinal explants derived from CD1 mice were fixed at various time points by immersion in cold 4% paraformaldehyde for 24 hours. Flat-mounted retinal explants were processed for immunofluorescent analysis at room temperature by washing twice in PBS, simultaneously permeabilizing and blocking using a solution of Triton X-100 (0.1%) and normal goat serum (5%) in PBS for 90 minutes, incubating with primary antibody (anti-β-III-Tubulin [Tuj1]; Neuromics, Edina, MN, USA) in blocking solution overnight, and incubating with secondary antibody...

Victoria Rozés Salvador, Florencia Heredia, Andrés Berardo, Anabela Palandri, Jose Wojnacki, Ana L. Vivinetto, Kazim A. Sheikh, Alfredo Caceres,& Pablo H.H. Lopez. Anti-glycan antibodies halt axon regeneration in a model of guillain Barrè syndrome axonal neuropathy by inducing microtubule disorganization via RhoA-ROCK-dependent inactivation of CRMP-2. Experimental Neurology. Available online 22 January 2016 doi:10.1016/j.expneurol.2016.01.016

...Primary antibodies (anti-TUJ1 (Neuromics) diluted 1:2000,...in blocking solution were added for 2 h at room temperature. ...

Lisa M. Mcginley, Erika Sims, J. Simon Lunn, Osama N. Kashlan, Kevin S. Chen, Elizabeth S. Bruno, Crystal M. Pacut, Tom Hazel, Karl Johe, Stacey A. Sakowski, Eva L. Feldman. Human Cortical Neural Stem Cells Expressing Insulin-Like Growth Factor-I: A Novel Cellular Therapy for Alzheimer's Disease. Stem Cells Trans Med January 2016 sctm.2015-0103 First Published Online January 7, 2016 doi: 10.5966/sctm.2015-0103

...To assess differentiation, cells were fixed with 4% paraformaldehyde(PFA), permeabilized with 0.1% Triton/phosphatebuffered saline (PBS), and blocked in 5% normal donkey serum per 0.1% Triton/PBS. Next,...TUJ1 (Neuromics, Edina, MN,http://www.neuromics.com),...primary antibodies were incubated at 1:1,000, unless otherwise indicated, overnight at 4°C. Cells were then incubated in Cy3, Cy5, or fluorescein isothiocyanate-conjugated secondary antibodies and mounted on glass slides usingProLong Gold antifade with 49,6-diamidino-2-phenylindole (DAPI)(Thermo Fisher Scientific). Images were captured using an Olympus BX-51 microscope (Olympus Corp.) and approximately 2.5 3 103 to 2.7 3 103 cells were counted per differentiation experiment for all samples (n = 3)...

Gaoying Sun, Wenwen Liu, Zhaomin Fan, Daogong Zhang, Yuechen Han, Lei Xu1, Jieyu Qi, Shasha Zhang, Bradley T. Gao, Xiaohui Bai,Jianfeng Li,Renjie Chai, Haibo Wang. The three-dimensional culture system with matrigel and neurotrophic factors preserves the structure and function of spiral ganglion neuron in vitro.

..Tuj-1-β-tubulin (1:1000, Neuromics, USA)...

Laura F. Corns, Lucy Atkinson, Jill Daniel, Ian J. Edwards, Lauryn New, Jim Deuchars and Susan A. Deuchars. Cholinergic Enhancement of Cell Proliferation in the Postnatal Neurogenic Niche of the Mammalian Spinal Cord. Article first published online: 26 JUN 2015 DOI: 10.1002/stem.2077.

...(beta tubulin III (Tuj1, chicken, 1:500 Neuromics, Edina, MN, http://www.neuromics.com/antibodies)...

Chowath, R. P. (2015) Role of Aurora kinase in Medulloblastoma development with correlation to MYCN activity (Dissertation). Available online. 

....Tuj1 (Neuron specific class 3 beta tubulin) [Neuromics]....

Ms. Amalia Gallegos-Cardenas, Dr. Robin L Webb, Erin T. Jordan, Ms. Rachel Claire West, Mr. Franklin D. West, Dr. Jeong Yeh Yang, Kai Wang, and Dr. Steve L Stice. Stem Cells and Development. -Not available-, ahead of print. doi:10.1089/scd.2015.0025.

...beta III-TUBULIN (βIII-TUB, Neuromics, 1:200)...

Guang Wang, Yan Li, Xiao-yu Wang, Manli Chuai, John Yeuk-Hon Chan, Jian Lei, Andrea Münsterberg, Kenneth Ka Ho Lee, and Xuesong Yang Mis-expression of BRE gene in the developing chick neural tube affects neurulation and somitogenesis. Mol. Biol. Cell 2015 : mbc.E14-06-1144v1-mbc.E14-06-1144.

...TuJ-1 (Neuromics 1:200)...

Nuno Jorge Lamas, Bethany Johnson-Kerner, Laurent Roybon, Yoon A. Kim, Alejandro Garcia-Diaz, Hynek Wichterle, Christopher E. Henderson. Neurotrophic Requirements of Human Motor Neurons Defined Using Amplified and Purified Stem Cell-Derived Cultures. Published: October 22, 2014DOI: 10.1371/journal.pone.0110324.

...chicken anti-β-III Tubulin (TUJ1, 1:1000, Neuromics)...

Loïc Binan, Charlène Tendey, Gregory De Crescenzo, Rouwayda El Ayoubi, Abdellah Ajji, Mario Jolicoeur. Differentiation of neuronal stem cells into motor neurons using electrospun poly-l-lactic acid/gelatin scaffold Biomaterials, Available online 22 October 2013.

...mouse anti-TUJ-1 (Neuromics, Edina, MN, MO15013)... http://dx.doi.org/10.1016/j.biomaterials.2013.09.097

Nathan P. Staff, Jewel L. Podratz, Lukas Grassner, Miranda Bader, Justin Paz, Andrew M. Knight, Charles L. Loprinzi, Eugenia Trushina, Anthony J. Windebank. Bortezomib alters microtubule polymerization and axonal transport in rat dorsal root ganglion neurons. NeuroToxicology, Volume 39, December 2013, Pages 124–131. http://dx.doi.org/10.1016/j.neuro.2013.09.001

...Neurons were incubated for 1 h at room temperature in 1:200 anti-Tuj-1 monoclonal antibody (Neuromics Antibodies, Edina, MN) to stain for beta-tubulin...

Dirk Puehringer, Nadiya Orel, Patrick Lüningschrör, Narayan Subramanian, Thomas Herrmann, Moses V Chao & Michael Sendtner. EGF transactivation of Trk receptors regulates the migration of newborn cortical neurons. Nature Neuroscience 16, 407–415 (2013) doi:10.1038/nn.3333

 ...Tuj-1 (Neuromics, MO15013, 1:1000)...

Mary J. Eaton, Yerko Berrocal, and Stacey Q. Wolfe. Potential for Cell-Transplant Therapy with Human Neuronal Precursors to Treat Neuropathic Pain in Models of PNS and CNS Injury: Comparison of hNT2.17 and hNT2.19 Cell Lines. Pain Research and Treatment. Volume 2012 (2012), Article ID 356412, 31 pages. doi:10.1155/2012/356412

...Modified methods for staining spinal cord sections for the human neuron-specific class III beta-tubulin (TuJ1) to identify grafted hNT2.19 neurons after grafting have previously been described [51]. The sections were washed with 0.1 M PBS pH 7.4 and permeabilized with 0.4% Triton-X-100 in 0.1 M PBS, 10% normal goat serum (NGS) for one hour. The sections were then incubated overnight at 4°C in the primary anti-TuJ1 antibody (1 : 100 DPBS), and the permeabilizing solution, followed by a one-hour incubation at room temperature with the secondary antibody solution, biotinylated mouse IgG raised in goat (Vector; 1 : 200), a Peroxidase ABC reporter in 0.1 M PBS (Vector) and “VIP” substrate (Vector). Some sections were stained in the absence of primary antibody and served as the negative controls...

Leanne M. Ramer, A. Peter van Stolk, Jessica A. Inskip, Matt S. Ramer and Andrei V. Krassioukov. Plasticity of TRPV1-expressing sensory neurons mediating autonomic dysreflexia following spinal cord injury. DOI=10.3389/fphys.2012.00257

For immunohistochemistry, slides were incubated in 10% normal donkey serum in PBS plus Triton X-100 (0.1%) for 20 min. We used five antibodies to delineate different subsets of nociceptors: these included antibodies raised against TRPV1 (Neuromics, Edina, MN, USA; 1:2,000)...Substance P (Neuromics, 1:1,000)...  β-III-tubulin (Neuromics; 1:500) – were used to label all neuronal profiles in the DRG.

Shinobu Hirai, Akiko Miwa, Chiaki Ohtaka-Maruyama, Masataka Kasai, Shigeo Okabe, Yutaka Hata and Haruo Okado. RP58 controls neuron and astrocyte differentiation by downregulating the expression of Id1–4 genes in the developing cortex. The EMBO Journal , (10 January 2012) | doi:10.1038/emboj.2011.486.

... anti-Tuj1(Neuromics, 1:500...

J. Simon Lunn, Crystal Pacut, Emily Stern, Stacey A. Sakowski, J. Matthew Velkey, Sue O'Shea, Eva Feldman. Intraspinal transplantation of neurogenin-expressing stem cells generates spinal cord neural progenitors. http://dx.doi.org/10.1016/j.nbd.2011.12.044.

...Tuj1 (Neuromics, 1:1000), Nestin (Neuromics, 1:500)...

Fadi J Najm, Anita Zaremba, Andrew V Caprariello, Shreya Nayak, Eric C Freundt, Peter C Scacheri, Robert H Miller & Paul J Tesar. Rapid and robust generation of functional oligodendrocyte progenitor cells from epiblast stem cells. Nature Methods (2011) doi:10.1038/nmeth.1712.

...βIII-tubulin (Tuj1; Neuromics)...

A. Young, D.W. Machacek, S.K. Dhara, P.R. MacLeish, M. Benveniste, M.C. Dodla, C.D. Sturkie and S.L. Stice. Ion channels and ionotrophic receptors in a human embryonic stem cell derived neural progenitors. doi:10.1016/j.neuroscience.2011.04.039.

...mouse anti nestin (neuromics), mouse anti tuj-1 (neuromics)...

C. Bettina Portmann-Lanz PhD, Andreina Schoeberlein PhD, Reto Portmann PhD, Stefan Mohr MD, Pierre Rollini PhD, Ruth Sager and Daniel V. Surbek MD. Turning placenta into brain: placental mesenchymal stem cells differentiate into neurons and oligodendrocytes. doi:10.1016/j.ajog.2009.10.893

...TuJ1 (1:500, Neuromics Edina).. β-Tubulin III (Tuj-1), Mouse, Neuromics...

Stephen I. Lentz, James L. Edwards, Carey Backus, Lisa L. McLean, Kristine M. Haines, and Eva L. Feldman. Mitochondrial DNA (mtDNA) Biogenesis: Visualization and Duel Incorporation of BrdU and EdU Into Newly Synthesized mtDNA In Vitro.  J. Histochem. Cytochem., Nov 2009; doi:10.1369/jhc.2009.954701. 

...Tuj1 (Neuromics Edina, MN)...

Alonso M. Higuero, Lucía Sánchez-Ruiloba1, Laura E. Doglio, Francisco Portillo1, José Abad- Rodríguez, Carlos G. Dotti and Teresa Iglesias. Kidins220/ARMS modulates the activity of microtubule-regulating proteins and controls neuronal polarity and development. JBC Papers in Press. Published on November 10, 2009 as Manuscript M109.024703.

...mouse monoclonal anti-beta III tubulin/tuj1 (MO15013, Neuromics Antibodies, Edina, MN)...

Shintaro Takahashi, Hironori Yamazoe, Fumihiro Sassa, Hiroaki Suzuki and Junji Fukuda. Preparation of coculture system with three extracellular matrices using capillary force lithography and layer-by-layer deposition. doi:10.1016/j.jbiosc.2009.06.013

...chicken IgY antibody against the neuronal marker Tuj1 (β-tubulin type Ш; Neuromics, Northfield, MN)...

Suzanne Timmons, Meghan F. Coakley, Aileen M. Moloney and Cora O’ Neill. Akt signal transduction dysfunction in Parkinson's disease. doi:10.1016/j.neulet.2009.09.055.

Ms. Amalia Gallegos-Cardenas, Dr. Robin L Webb, Erin T. Jordan, Ms. Rachel Claire West, Mr. Franklin D. West, Dr. Jeong Yeh Yang, Kai Wang, and Dr. Steve L Stice. Stem Cells and Development. -Not available-, ahead of print. doi:10.1089/scd.2015.0025.

...beta III-TUBULIN (βIII-TUB, Neuromics, 1:200)...

...β-III-tubulin (1:1000) and neuron specific enolase (1:1000) Neuromics...  

Vidya Murthy, Stéphane F. Maison, Julián Taranda, Nadeem Haque, Chris T. Bond , A. Belén Elgoyhen, John P. Adelman, M. Charles Liberman, Douglas E. Vetter. SK2 channels are required for function and long-term survival of efferent synapses on mammalian outer hair cells. Molecular and Cellular Neuroscience 40 (2009) 39–49

...TuJ-1 (class III β-tubulin, Neuromics, Northfield, MN; cat. # MO15013) and processedwith Oregon Green labeled secondary antibodies (Molecular Probes/InVitrogen) for confocal microscopy...

Yoshifumi Saisho, Paul E. Harris, Alexandra E. Butler, Ryan Galasso, Tatyana Gurlo,1 Robert A. Rizza, and Peter C. Butler. Relationship between pancreatic vesicular monoamine transporter 2 (VMAT2) and insulin expression in human pancreas. J Mol Histol. 2008 October; 39(5): 543–551. Published online 2008 September 13. doi: 10.1007/s10735-008-9195-9.

Sujoy K. Dhara, Kowser Hasneen, David W. Machacek, Nolan L. Boyd, Raj R. Rao, Steven L. Stice (2008). Human neural progenitor cells derived from embryonic stem cells in feeder-free cultures. Differentiation 76 (5) , 454–464 doi:10.1111/j.1432-0436.2007.00256.x

...NES (1:100, Neuromics, Edina, MN), MSI1 (1:100, Neuromics), Tuj1 (1:500, Neuromics)...

D. Conte, V. Lall, G. Dobson, S. Deuchars, J. Deuchars. Cerebrospinal fluid contacting neurones in the spinal cord of the mouse and rat: small cells with a big purpose? University of Leeds (2008) Proc Physiol Soc 10 PC45

...Preliminary immunohistochemical data supports the concept that the CSFcNs are immature neurones, since they express neuronal markers Tuj1 (Neuromics) ...