Shengnan Zhao, Zizhen Gong1, Jiefei Zhou1, Chunyan Tian, Yanhong Gao, Congfeng Xu, Yingwei Chen, Wei Cai and Jin Wu1Deoxycholic Acid Triggers NLRP3 Inflammasome Activation and Aggravates DSS-Induced Colitis in Mice. Front. Immunol., 28 November 2016 | http://dx.doi.org/10.3389/fimmu.2016.00536

...Lipopolysaccharide-primed J774A.1 cells were incubated with or without DCA (100 μM, 24 h); then, the cells were stained with Lyso Tracker Green DND-26 (Invitrogen) or magic red cathepsin B fluorogenic substrate z-Arg-Arg cresyl violet (Neuromics) for 1 h, followed by Hoechst staining for half an hour. Fluorogenic signals were captured by inverted fluorescence microscope (Leica)...

Florian M. Baumann, Yevgeniy Yuzefpolskiy, Surojit Sarkar, Vandana Kalia. Dicer Regulates the Balance of Short-Lived Effector and Long-Lived Memory CD8 T Cell Lineages. Published: September 14, 2016. dx.doi.org/10.1371/journal.pone.0162674.

...For caspase staining, the FAM-FLICA Apoptosis Detection Kit (Caspase 3 & 7 Assay Kit, green) was used (Neuromics, Minneapolis, MN). Cells were stained according to the manufacturer’s protocol in a 96-well plate, using 20 min incubation at 37°C together with CD8 PE-Cy7, Thy1.1 Alexa 700, and Thy1.2 Pac Blue antibodies...

Vitor Y. R. Soares, Nadia A. Atai, Takeshi Fujita, Sonam Dilwali, Sarada Sivaraman, Lukas D. Landegger, Fred H. Hochberg, Carlos A. P. C. Oliveira, Fayez Bahmad, Xandra O. Breakefield and Konstantina M. Stankovic. Extracellular vesicles derived from human vestibular schwannomas associated with poor hearing damage cochlear cells. Neuro Oncol (2016) doi: 10.1093/. First published online: May 18, 2016

Venkata Kashyap Yellepeddi, Kiran Kumar Vangara, Srinath Palakurthi. Poly(amido)amine (PAMAM) dendrimer–cisplatin complexes for chemotherapy of cisplatin-resistant ovarian cancer cells. Journal of Nanoparticle Research. August 2013, 15:1897. 10.1007/s11051-013-1897-6.

...FAM-FLICATM apoptosis detection kit (Neuromics, Minneapolis, MN, USA)...

Minghai Zhou, Gregory Ottenberg, Gian Franco Sferrazza, and Corinne Ida Lasmézas Highly neurotoxic monomeric α-helical prion protein. PNAS 2012 109 (8) 3113-3118; published ahead of print February 7, 2012, doi:10.1073/pnas.1118090109.

...PFA and stained for caspases using FLICA polycaspase assay (Neuromics) according to the manufacturer's instructions. Caspases...assayed on live cells using the caspase- specific FLICA assay (Neuromics). Cells were photographed using a Nikon inverted epifluorescence...

Giovanna Grandinetti, Nilesh P. Ingle, and Theresa M. Reineke. Interaction of Poly(ethylenimine)–DNA Polyplexes with Mitochondria: Implications for a Mechanism of Cytotoxicity. Mol. Pharmaceutics, Article ASAP Publication Date (Web): June 23, 2011 Copyright © 2011 American Chemical Society

...inhibitor of caspases (FLICA) specific for caspase-9 (Neuromics, Inc)...

M M Lovric and C J Hawkins. TRAIL treatment provokes mutations in surviving cells. Oncogene advance online publication 19 July 2010; doi: 10.1038/onc.2010.242.

...Cells were washed in PBS again three times then incubated in anti-rabbit FITC antibody 1:200 for 2 h in the dark. Cells were washed with PBS containing 1% bovine serum albumin for three times and 1:30 SR-DEVD-FMK reagent (Neuromics) was added and incubated at 37 °C, 5% CO2 for 1 h in the dark...

L. Wei, D. Ding and R. Salvi. Salicylate-induced degeneration of cochlea spiral ganglion neurons-apoptosis signaling. doi:10.1016/j.neuroscience.2010.03.015.

...A Polycaspase Assay Kit (Green, Neuromics, KF17200)...

Aaron J. Thomas and Carol A. Erickson. FOXD3 regulates the lineage switch between neural crest-derived glial cells and pigment cells by repressing MITF through a non-canonical mechanism. Development, Apr 2009; doi:10.1242/dev.031989.

...Apoptosis was assayed using Magic Red Caspases 3&7 reagent (Neuromics)...