Lena Willkomm, Sebastian Gehlert, Daniel Jacko, Thorsten Schiffer, Wilhelm Bloch. p38 MAPK activation and H3K4 trimethylation is decreased by lactate in vitro and high intensity resistance training in human skeletal muscle. PLOS. Published: May 3, 2017https://doi.org/10.1371/journal.pone.0176609

...Immunofluorescence (IF) of cells

Cells were fixed by incubation with 4% PFA in DPBS for 10 min at the before mentioned time points. Cells were stained as described in [4]. Briefly, cells were permeabilized with a 0.5 M ammonium chloride solution in PBS containing 0.25% Triton X-100. After washing, unspecific binding sites were blocked with 5% BSA in PBS for 1 h at room temperature. Cells were then incubated with primary antibodies H3K4me3 (rabbit; dilution 1:1000; Cell Signaling Technology, Danvers, MA, USA), Pax7 (mouse; dilution 1:250; Neuromics, Edina, NM, USA), Myf5 (rabbit; 1:250; Genetex, Irvine, CA, USA, F5D (myogenin; mouse; dilution 1:250), or Mf20 (myosin heavy chain; mouse; dilution 1:500; both Developmental Studies Hybridoma Bank, Iowa City, IO, USA) in 0.8% BSA in PBS at 4°C overnight. The following day, the primary antibody solutions were washed off and cells incubated with a secondary antibody solution containing Alexa488-conjugated goat-anti-mouse IgG or Alexa488-conjugated goat-anti-rabbit IgG (dilution 1:1000; both from Invitrogen, Karlsruhe, Germany) for 1 h at room temperature. This solution was again washed off and 5 min incubation with DAPI (Sigma-Aldrich, Steinheim, Germany) followed before cells were washed for the last time and cover slips mounted onto microscopic slides using Aqua- Poly/Mount (Polysciences, Warrington, PA, USA)....

Abigail L. Mackey, Lotte K. Rasmussen, Fawzi Kadi, Peter Schjerling, Ida C. Helmark, Elodie Ponsot, Per Aagaard, João Luiz Q. Durigan and Michael Kjaer. Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti-inflammatory medication. FASEB Journal. Published online before print March 2, 2016, doi:10.1096/fj.201500198R

... peroxidase. In brief, satellite cells were labeled with a mouse anti-Pax7 antibody(Neuromics, Edina, MN, USA)...

Hoedt, A., Christensen, B., Nellemann, B., Mikkelsen, U. R., Hansen, M., Schjerling, P. and Farup, J. (2015), Satellite cell response to erythropoietin treatment and endurance training in healthy young men. J Physiol. Accepted Author Manuscript. doi:10.1113/JP271333

...Sections were then incubated overnight at 4°C with primary antibodies against Pax7 (1:500; Cat no MO15020, Neuromics, Edina, MN...

Jean Farup, Michael De Lisio, Stine Klejs Rahbek, Jonas Bjerre, Mikkel Holm Vendelbo, Marni D. Boppart, Kristian Vissing. Pericyte Response to Contraction Mode-Specific Resistance Exercise Training in Human Skeletal Muscle. Journal of Applied Physiology Sep 2015, DOI: 10.1152/japplphysiol.01108.2014

...sections were incubated at 4°C overnight with the primary antibodies against Pax7 (1:500, Cat no MO15020, Neuromics) ...

Lena Willkomm, Sarah Schubert, Raphael Jung, Manuela Elsen, Julika Borde, Sebastian Gehlert, Frank Suhr, Wilhelm Bloch. Lactate regulates myogenesis in C2C12 myoblasts in vitro. Stem Cell Research, Volume 12, Issue 3, May 2014, Pages 742-753

Note: PAX7 used for WB and ICC on Myoblasts in Culture and Cell Lysates

Saclier, M., Yacoub-Youssef, H., Mackey, A. L., Arnold, L., Ardjoune, H., Magnan, M., Sailhan, F., Chelly, J., Pavlath, G. K., Mounier, R., Kjaer, M. and Chazaud, B. (2013), Differentially Activated Macrophages Orchestrate Myogenic Precursor Cell Fate During Human Skeletal Muscle Regeneration. STEM CELLS, 31: 384–396. doi: 10.1002/stem.1288

...Pax7 1/100 (MO15020; Neuromics, Edina, MN...

Nicklas S , Otto A , Wu X , Miller P , Stelzer S , et al. 2012 TRIM32 Regulates Skeletal Muscle Stem Cell Differentiation and Is Necessary for Normal Adult Muscle Regeneration. PLoS ONE 7(1): e30445. doi:10.1371/journal.pone.0030445.

...Pax7 (Neuromics, 1:100)...

Abigail Louise Mackey, Lars L. Andersen, Ulrik Frandsen, Charlotte Suetta, and Gisela Sjogaard. Distribution of myogenic progenitor cells and myonuclei is altered in women with vs. those without chronically painful trapezius muscle. J Appl Physiol (October 7, 2010). doi:10.1152/japplphysiol.00789.2010.

...PAX7 (MO15020; Neuromics, Edina, Minnesota)...

Ulla Ramer Mikkelsen, Henning Langberg, Ida Caroe Helmark, Dorthe Skovgaard, Lars L. Andersen, Michael Kjaer, and Abigail Louise Mackey.Region specific prostaglandin blockade inhibits satellite cell proliferation in human skeletal muscle following eccentric exercise. J Appl Physiol, Aug 2009; doi:10.1152/japplphysiol.00707.2009.

...Pax7 mouse Satellite cells Neuromics, Edina, Minnesota, USA MO15020 1:100...