Several varieties of Cas9 have been studied and there appear to be several other related enzymes with similar properties in bacteria. Two Cas9 homologs include Streptococcus pyogense and Staphylococcus aureus. Staphylococcus aureus is significantly smaller and so presents less problems when packaged into vectors. The S. pyogenes protein is rather large at 1,368 amino acids, ~160kDa, so the corresponding DNA is also rather large at about 4.2 kb.
Image: Transfected Hek293 cells overexpressing the N-terminal 1-608 amino acids of S. pyogenes Cas9 . The cells were stained with MO22174 in red, and these cells also appear yellow since we counterstained with our rabbit Cas9 antibody, RA22126 to the same construct in green, giving a yellow color. The N-terminal construct contains a nuclear localization sequence and so is predominantly nuclear in localization. Most Hek293 cells in this field are not transfected so only the nuclei of these cells can be visualized with the blue DAPI DNA stain.
|Cas9 from Steptococcus pyogenes||MO22174||Mouse IgG||Not Applicable||ICC; IF; WB||100 ul||$295|
|Cas9 from S. pyogenes||RA22126||Rabbit IgG||Not Applicable||ICC; IF; WB||100 ul||$295|
|Cas9 from Staphylococcus aureus||MO22175||Mouse IgG||Not Applicable||ICC; IF; IHC; WB||100 ul||$295|
|Cas9 from Staphylococcus aureus||RA22127||Rabbit IgG||Not Applicable||ICC; IF; IHC; WB||100 ul||$295|
|Cas9 from Staphylococcus aureus||CH22120||Chicken IgY||Not Applicable||ICC; IF; IHC; WB||100 ul||$295|