5HT (Serotonin) 2A Receptor, 5HT2A R

Affinity Purified Rabbit Antibody
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Type: Rabbit IgG
 
Applications: IHC; WB
ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; WB=Western blotting; FC=Flow Cytometry; IP=Immunoprecipitation; E=ELISA; NB=Neutralization of Bioactivity; FACS; FM=Fluorescent Micsroscopy; ; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; TPE=Targeted Protein Expression; ; ; AC=Adherent Cell Assays; ; ; NAC=Non-adherent Cell Assays; ; ; BSC-CM5= Biacore Sensor Chip CM5; ;
Species Reactivity: R
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins
Format: Affinity Purified - liquid
 
Immunogen: multiple antigenic peptide of an N-terminal synthetic sequence corresponding to amino acids 22-41 of rat 5HT2A receptor
 
Description/Data:
Picture

The affinity purified histochemical antibody for 5HT2A receptor was generated in rabbit against a multiple antigenic peptide of an N-terminal synthetic sequence corresponding to amino acids 22-41 of rat 5HT2A receptor.

Image: 5HT2AR staining of rat cerebral cortex.

The antibody produces 4+ (maximum) immunolabeling of 5HT2A receptor at dilutions of 1/300 - 1/500 using biotin-streptavidin/HRP technique with free-floating methods on rat cortex, amygdala and hippocampus. The addition of intensifying reagents such as nickel ammonium sulfate to the chromogen solution allows further dilution to approximately 1/1,000 or more. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. Immunolabeling is completely abolished by preadsorption with synthetic rat 5HT2A receptor (22-41). Immunolabeling of Western blot revealed a single band of approximately between 55 and 75 kDA. For Western Blot Protocol See: DOI:10.1124/jpet.111.18378.

5HT2AR: Customer Publications

More Links:

All 5HT-Serotonin Antibodies

Neurotransmission Research Antiboodies

Primary Neurons and Astrocytes -Primary human, rat and mouse neurons and astrocytes

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Image: Mouse frontal cortical neuron cultures (p1 pups, day 4 culture stained-Neuromicsantibody: 5-HT2AR at 1:100 48 hours. MAP2 Ab: 1:20,000, 24 h. Details in Schmid, Raehal, and Bohn; 2008; PNAS.