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The affinity purified histochemical antibody for 5HT2A receptor was generated in rabbit against a multiple antigenic peptide of an N-terminal synthetic sequence corresponding to amino acids 22-41 of rat 5HT2A receptor.
Image: 5HT2AR staining of rat cerebral cortex.
The antibody produces 4+ (maximum) immunolabeling of 5HT2A receptor at dilutions of 1/300 - 1/500 using biotin-streptavidin/HRP technique with free-floating methods on rat cortex, amygdala and hippocampus. The addition of intensifying reagents such as nickel ammonium sulfate to the chromogen solution allows further dilution to approximately 1/1,000 or more. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. Immunolabeling is completely abolished by preadsorption with synthetic rat 5HT2A receptor (22-41). Immunolabeling of Western blot revealed a single band of approximately between 55 and 75 kDA. For Western Blot Protocol See: DOI:10.1124/jpet.111.18378.
5HT2AR: Customer Publications
Image: Mouse frontal cortical neuron cultures (p1 pups, day 4 culture stained-Neuromicsantibody: 5-HT2AR at 1:100 48 hours. MAP2 Ab: 1:20,000, 24 h. Details in Schmid, Raehal, and Bohn; 2008; PNAS.