|Catalog Number||Size||Price (USD)||Shopping Cart|
|MO22117||100 ul||$225.00 $275.00||Buy Now | Add to Cart|
Tar DNA Binding Protein, TDP43 or TARDBP was originally identified as a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. It was also reported to regulate alternate splicing of the CFTR gene and the apoA-II gene.
Image: TDP43 staining of formalin fixed adult rat brain, specifically the hippocampus. Hippocampal neuron nuclei are stained strongly. Chicken antibody to GFAP (green) shows the processes of astrocytic glial cells. Nuclei of all cells are revealed with DAPI DNA stain (blue). The TARDP antibody stains neuronal nuclei strongly and the nuclei of some non-neuronal cells much more weakly. Neuronal nuclei therefore look crimson, since they are both red due to the content of TDP43 and blue due to their content of DNA, stained blue with DAPI. Protocol on data-sheet.
Later it was discovered that hyper-phosphorylated, ubiquitinated and cleaved form of TARDBP, known as pathologic TDP43, is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in Amyotrophic lateral sclerosis (ALS). Elevated levels of the TDP-43 protein have also been identified in individuals diagnosed with chronic traumatic encephalopathy, a condition that often mimics ALS and that has been associated with athletes who have experienced multiple concussions and other types of head injury.
Primary Neurons and Astrocytes-Primary human, rat and mouse neurons and astrocytes
Images; Blots of TDP43 on crude extract of mouse brain nuclear fraction (left lane) and cytoplasmic fraction (right lane). There is a strong clear band in the nuclear preparation running at 43kDa, and a much more minor band in the cytoplasmic fraction. Image courtesy of Irina Madorsky. Protocol on data-sheet.