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Type: Guinea Pig IgG
Applications: ICC; IHC
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: R
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Whole serum - liquid

The acid-sensing ion channel family (ASIC) comprises six discrete ASIC subunits (ASIC 1A, ASIC 1B, ASIC 2A, ASIC 2B, ASIC 3, ASIC 4) that detect tissue acidosis (i.e. a decrease in pH). This acidosis phenomenon is a  response to tissue injury, pain and inflammation.

Figure: ASIC3 expression compared to other nociceptive ion channels. DRG sections double-labeled for ASIC3 (red) and either P2X3 (green, A) or TRPV1 (green, B); double-labeled cells are yellow or orange. Circle charts show the relative distribution of the three receptors among all DRG neurons. ASIC3 and P2X3 expression overlapped in only 4% of neurons, while some 35% of ASIC3-positive neurons also express TRPV1. The rare cells positive for both ASIC3 and P2X3 were large (eg. cell at lower left in A). 1406 cells counted for A; 995 for B. C, D, electrophysiological recordings reveal a qualitatively similar expression pattern for currents conforming to ASIC3, P2X3, or TRPV1. The cell that generated the currents in C counts as a co-expressor of ASIC3 and P2X3, fitting into the yellow bin in the circle plot. The cell in D is a co-expressor of ASIC3 and TRPV1. Cells were counted positive if they had at least 0.3 nA of current in response to pH 6.8 (ASIC3+), 50 μM ATP (P2X3+; transient current only), or 1 μM capsaicin (TRPV1). 182 cells recorded for C; 169 for D.

ASIC3: Customer Publications


Images: ASIC3 (Dilution 1:10) amd P2X3 (Dilution 1:500) satining of rat Dorsal Root Ganglia (DRGs) of cisplatin-treated animals. After dilution in 0.1 M phosphate-buffered saline (PBS) containing 1.5% normal goat serum and 0.3% Triton X-100 (Sigma), DRG sections were incubated with either guinea pig polyclonal antiserum against synthetic rat ASIC3 and rabbit polyclonal antiserum against synthetic rat P2X3 (1:500; Neuromics). The sections for ASIC3 were reacted with reagents for 2 days at room temperature and others at 4 C. After being rinsed with 0.1 M PBS, the sections were reacted in PBS with fluorescein-isothiocyanate (FITC)-conjugated goat anti-guinea pig or - rabbit IgG antibody (Vector Laboratories, Burlingame, CA, USA) at a concentration of 1:100. After being rinsed with 0.1 M PBS, the sections were cover-slipped in mounting medium (Immunon, Pittsburgh, PA, USA) and examined under a fluorescence microscope equipped with a digital camera. (see: PAIN 149 (2010) 393–405).


Image: Detection of ASIC3 expression in normal and CF human lung tissues by immunofluorescence microscopy. Normal (top panels A-C) and CF human lung sections (bottom panels D-F) were labeled with a specific antibody against ASIC3 (green channel) and the DNA-selective Hoechst dye (blue channel). A and D, normal and CF human lung sections imaged following incubation with the mixture of anti-ASIC3 antibody and immunopeptide. Shown are normal (B) and CF (E) alveolar structures at a small magnification. ASIC3 expression was detected in both types I and II alveolar cells. Shown are normal (C) and CF (F) alveolar structures at a large magnification. Scale bars equal 10 µm in A, B, D, and E and 20 µm in C and F. These representative images were from 11 sections of four CF lungs. J. Biol. Chem., Vol. 281, Issue 48, 36960-36968, December 1, 2006