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CH23019200 ul$295.00Buy Now | Add to Cart
 
Type: Chicken IgY
 
Applications: IHC
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: M
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Affinity Purified - liquid
 
Immunogen: Recombinant mouse PAP protein was expressed using a baculoviral-delivery system.
 
Description/Data:
Picture

Mouse PAP is a 43,698 dalton protein (381 amino acids; NCBI accession numberAAF23171) associated with prostatic cancer cells, as well as primary afferent sensory neurons involved in the pain pathway. This protein is an enzyme that dephosphorylates adenosine monophosphate (AMP) in the dorsal horn gray matter of the spinal cord, generating free adenosine. Injections of PAP into the dorsal horn of experimental mice has been shown to decrease pain perception by acting in an antinociceptive, antihyperalgesic, and antiallodynic fashion.

Images: Tissue sections through a 10%-formalin fixed adult mouse. Sections were examined for PAP positive dorsal root ganglion sensory neurons. Secondary antibodies (fluorescein-labeled goat anti-chicken IgY, Neuromics Cat. #CH23101)  were used at a concentration of 1:500.

This makes PAP is an excellent marker for studying the neurotransmission of nociceptive and neuropathic pain.