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MO15013100 ug$265.00Buy Now | Add to Cart
Type: Mouse IgG
Applications: ICC; IF; IHC; WB
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Protein G Purified - liquid
Immunogen: Microtubules derived from rat brain

Human β-Tubulin 3 is a 50,432 dalton structural protein (450 amino acid) expressed in neurons of the PNS and CNS. It contributes to microtubule stability in neuronal cell bodies and axons, and plays a role in axonal transport.

Image: Tuj-1 staining of Neuron-specific class III β-tubulin in differentiated human neural progenitor cells. Cells were stained using goat anti-mouse Alexa Fluor 488 (green) secondary antibody (Molecular Probe, A-11001) and counterstained with PI (red).


Image: Neural progenitors were labeled with anti-rat Nestin polyclonal antibody (Cat#:GT15114) and stained with conjugated donkey anti-goat secondary anti-body (green). Differentiated neurons were labeled with neuron-specific mouse anti-β-III tubulin/ Tuj1-(Cat#MO15013 monoclonal antibody (red). Nuclei were stained with DAPI (blue).

Tuj-1 Customer IHC Protocol

Tuj-1: Customer Publications and Data 



Images: Immunostaining of  hNP1 Progenitors before and after differentiation. The culture was highly homogenous with neural rosettes. (A) Neural rosettes (white arrows, bright field) from WA09 cells. A similar result also obtained with BG02 cell line. (B) Shown here, a neural rosette stained with Nestin (NES) (green) antibody. Propagated Neural progenitors showed expression of marker genes, NES (C) and Musashi 1 (D) but not SOX2 (E). Further differentiation produced neurons (Tuj1) (F), astrocytes (GFAP) (G) and oligodendrocytes (myelin basic protein)(H) (lower panel). DAPI (blue) was used for staining the nuclei (scale bar for (A) through (E) is 100 mm and for the remaining figures 10 mm). Differentiation (2007) DOI: 10.1111/j.1432-0436.2007.00256.x...Dilutions: NES (1:100, Neuromics, Edina, MN), MSI1 (1:100, Neuromics), Tuj1 (1:500, Neuromics)...