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MO15052100 ul$255.00Buy Now | Add to Cart
 
Type: Mouse IgG
 
Applications: IHC; WB; E
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H; R
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Protein G Purified - liquid
 
Immunogen: Hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, E. coli-derived recombinant human Glial Fibrillary Acidic Protein (rhGFAP; aa 292 - 432; Accession # P14136)..
 
Description/Data:
Picture

This antibody reacts with the 52 kD intermediate filament protein GFAP in brain and spinal cord. It labels some astrocytes and some CNS ependymal cells but not oligodendrocytes or neurons. This antibody does not react with other intermediate filament proteins

Image: Rat cortical stem cells were differentiated for 7 days and stained with GFAP (red) and Tuj-1 ( green). The nuclei were stained with DAPI (blue)

Immunohistochemistry Protocol: This antibody was used at a concentration of 5 - 10 μg/mL to detect human GFAP in astrocytes differentiated from neural progenitors. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.3% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4° C followed by Rhodamine Red™-coupled anti-mouse IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. GFAP: Customer Publications