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GT15232100 ug$365.00Buy Now | Add to Cart
 
Type: Goat IgG
 
Applications: ICC; WB
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: H; M
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Affinity Purified - liquid
 
Immunogen: E. coli derived recombinant mouse SLAIN1 Met262-Gln483 Accession # Q68FF7.
 
Description/Data:
Picture

SLAIN1 (SLAIN motifcontaining protein 1) is a 61 kDa (predicted) member of the SLAIN motif containing family of proteins. It is expressed in embryonic stem cells plus cells of the neural tube, and also appears in multiple adult tissues. Its function is unclear. Mouse SLAIN1 is 579 amino acids (aa) in length. It contains a coiledcoil region (aa 2156), a Prorich segment (aa 64160), and one Cterminal SLAIN (SerLeuAlaIleAsn) motif. There are two potential splice variants. One shows an alternate start site at Met262, while another shows a 50 aa insertion after Gln231. Over aa 262483, mouse SLAIN1 shares 96% and 92% aa identity with rat and human SLAIN1, respectively.

Image: SLAIN1 staining of  immersion fixed D3 mouse embryonic stem cell line (dilution of 10 μg/mL  for 3 hours at room temperature). Cells were stained with the 557 fluoro-labeled conjugated AntiGoat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Protocol on Datasheet..

Picture[2]

Image: Detection of Human/Mouse SLAIN1 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, human cerebellum tissue, and BG01V human embryonic stem cells. PVDF membrane was probed with 1 μg/mL of Human/Mouse SLAIN1 Antibody followed by HRPconjugated AntiGoat IgG Secondary Antibody. A specific band was detected for at approximately 60 kDa (as indicated). Protocol on Datasheet..