|Catalog Number||Size||Price (USD)||Shopping Cart|
|GT15239||100 ug||$365.00||Buy Now | Add to Cart|
5T4 (oncofetal antigen 5T4; also TPBG and trophoblast glycoprotein) is a 72 - 80 kDa glycoprotein member of the LRR family of proteins. It is expressed on trophoblasts, tumor cells, ovarian cuboidal epithelium and differentiating embryonic stem cells and impacts cell adhesion and motility. The mouse 5T4 cDNA encodes a type I transmembrane protein precursor that is 426 amino acids (aa) in length. It contains a 332 aa extracellular region (aa 30 - 361) that shows one Ser-rich region followed by seven Leu-rich repeats (aa 90 - 338). Over aa 30 - 361, mouse 5T4shares 95% and 81% aa identity with rat and human 5T4, respectively.
Image: 5T4 staining of Differentiated Mouse D3 Embryonic Stem Cells. Mouse D3 embryonic stem cells grown in the presence of serum, without LIF or a fi broblast feeder cell layer Celle were stained with 557 fluoro-labeled-conjugated anti-sheep secondary antibody (red). Nuclei were counterstained with DAPI (blue).
Right image: Detection of 5T4 by western blot.Mouse brain lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0 μg/mL of antibody.
Left image: Differentiated D3 cells were stained with anti-5T4 (filled histogram)