Type: Goat IgG
Applications: IHC; WB; E
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: M
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Affinity Purified - liquid
Immunogen: Purified, E. coli-derived, recombinant mouse Stella.
5T4 (oncofetal antigen 5T4; also TPBG and trophoblast glycoprotein) is a 72 - 80 kDa glycoprotein member of the LRR family of proteins. It is expressed on trophoblasts, tumor cells, ovarian cuboidal epithelium and differentiating embryonic stem cells and impacts cell adhesion and motility. The mouse 5T4 cDNA encodes a type I transmembrane protein precursor that is 426 amino acids (aa) in length. It contains a 332 aa extracellular region (aa 30 - 361) that shows one Ser-rich region followed by seven Leu-rich repeats (aa 90 - 338). Over aa 30 - 361, mouse 5T4shares 95% and 81% aa identity with rat and human 5T4, respectively.
Image: 5T4 staining of Differentiated Mouse D3 Embryonic Stem Cells. Mouse D3 embryonic stem cells grown in the presence of serum, without LIF or a fi broblast feeder cell layer Celle were stained with 557 fluoro-labeled-conjugated anti-sheep secondary antibody (red). Nuclei were counterstained with DAPI (blue).