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SSEA3 (stage-specific mouse embryonic antigen) is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis.
Image: SSEA3 staining of HeLa cells (Green) detected with Fluor 488 conjugated anti-rat IgM secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue). Protocol on Datasheet.
The SSEA3 protein is present on the surface of human teratocarcinoma cells, embryonic germ cells, and embryonic stem cells but not mouse undifferentiated mouse cells of these types
Images: FACS staining of NTERA-2 cells using SSEA3 at a 1:50 dilution detected using 488 conjugated goat anti-rat IgM secondary antibody.