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Otx 2 is an early marker of endodermal differentiation of embryonic stem cells. This includes midbrain dopaminergic (mDA) neuronal progenitors (NPs) arise from the ventral mesencephalic area. see: http://www.pnas.org/content/108/23/9703
Image: Characterization of the human neuroectodermal precursors. Otx2 Staining of forebrain-midbrain rosettes (dilution 1:1000).
Otx-2: Customer Publications
Image: Immunocytochemistry on FACS-puriﬁed mDompaminergic NPs using anti-Corin antibody and anti-Otx2 antibody. http://www.pnas.org/content/108/23/9703