Neurofilaments or NFs are the 10 nanometer (10nm) or intermediate filaments found specifically in neurons. During axonal growth, new neurofilament subunits are incorporated all along the axon in a dynamic process that involves the addition of subunits along the filament length, as well as the addition of subunits at the filament ends. After an axon has grown and connected with its target cell, the diameter of the axon may increase as much as fivefold. Neurofilaments are repulsive. This is because their purpose is to set the diameter of dendrites and axons. They do this by repelling each other because of their polarity and move away from each other. The level of neurofilament gene expression seems to directly control axonal diameter, which in turn controls how fast electrical signals travel down the axon. Neurofilament markers include: neurofilament light (NFL), neurofilament medium (NFM) and neurofilament heavy (NFH). They may also include peripherin, alpha-internexin, nestin and in some cases vimentin.

Images: Cells grown from adult rat brainLarge cell in middle is stained with mouse monoclonal to NF-L clone DA2 (green). Another type of neuronal lineage cell was stained with rabbit polyclonal to alpha-internexin (red). These cells were mitotic but had several characteristics of neurons. Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including: NF-H, NF-M,, NF-L,, NF66 and GFAP .

These antibodies are excellent markers for adult and developing neurons including neural progenitors. They are also useful for the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.

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NameCatalog #TypeSpeciesApplicationsSizePrice
NestinMO15056Mouse IgGM; RFC; ICC; IHC100 ug$255
NestinMO15012Mouse IgGH; PrFC; ICC; IHC100 ug$265
NestinCH23001Chicken IgYH; M; RICC; IHC; WB100 ul$99
NestinGT15114Goat IgGM; RIHC; WB; E100 ug$365
NestinRA22125Rabbit IgGHICC; IF; IHC; WB100 ul$295
Neurofilament NF-HCH22104Chicken IgYH; M; Pr; RICC; IHC; WB50 ul$275
Neurofilament NF-HCH23015Chicken IgYH; MIHC200 ul$285
Neurofilament NF-HMO22120Mouse IgGB; Ca; Ch; D; EQ; GP; H; M; P; Pr; R; RbICC; IF; IHC; WB100 ul$275
Neurofilament NF-H, phosphate independentRA22112Rabbit IgGRIF; WB100 ul$275
Neurofilament NF-HMO22103Mouse IgGCh; H; M; Pr; RICC; IHC; WB100 ul$225
Neurofilament NF-H, phosphylatedRA22116Rabbit IgGCh; H; M; Pr; RICC; IHC; WB100 ul$275
Neurofilament NF-LCH22105Chicken IgYCa; Ch; H; M; RICC; IHC; WB100 ul$275
Neurofilament NF-LMO22104Mouse IgGCa; Ch; H; M; RICC; IHC; WB; E100 ul$275
Neurofilament NF-LRA22104Rabbit IgGCa; Ch; H; M; RICC; IF; WB100 ul$275
Neurofilament NF-MCH22106Chicken IgYCh; H; M; RICC; IHC; WB100 ul$275
Neurofilament NF-MMO22105Mouse IgGCh; H; M; RICC; IHC; WB100 ul$225
Neurofilament NF-MCH23014Chicken IgYH; MIHC200 ul$285
Neurofilament NF-MRA22105Rabbit IgGCa; Ch; H; M; RICC; IHC; WB100 ul$275
Neurofilament alpha-internexin/NF66MO25017Mouse IgGH; RICC; IF; WB500 ul$275
Neurofilament alpha-internexin/NF66CH22101Chicken IgYH; M; RFC; IF100 ul$275
Neurofilament alpha-internexin/NF66RA22102Rabbit IgGH; M; RIF; WB100 ul$275
PeripherinCH22111Chicken IgYCa; H; M; RIHC100 ul$245
PeripherinCH23016Chicken IgYH; MIHC200 ul$285
PeripherinRA22109Rabbit IgGCa; H; M; RICC; WB100 ul$275
PeripherinMO22106Mouse IgGCa; H; M; RICC; IF; WB500 ul$275
VimentinCH23010Chicken IgYH; MICC; IHC; WB100 ul$99
VimentinCH22108Chicken IgYH; M; RIHC; WB100 ul$275