Neuron Specific Enolase (NSE)/ENO2

Whole Serum Rabbit Antibody
DATASHEET(pdf - 123Kb)
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Type: Rabbit IgG
 
Applications: IF; WB
ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; WB=Western blotting; FC=Flow Cytometry; IP=Immunoprecipitation; E=ELISA; NB=Neutralization of Bioactivity; FACS; FM=Fluorescent Micsroscopy; ; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; TPE=Targeted Protein Expression; ; ; AC=Adherent Cell Assays; ; ; NAC=Non-adherent Cell Assays; ; ; BSC-CM5= Biacore Sensor Chip CM5; ;
Species Reactivity: H
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins
Format: Whole serum - liquid
 
Immunogen: Purified recombinant full length human NSE protein from E. coli.
 
Description/Data:
Picture

Human Neuron specific enolase (NSE) (EC 4.2.1.11) is a 47,138 dalton protein (434 amino acids) expressed in neurons of the peripheral nervous system (PNS) and central nervous system (CNS). NSE catalyzes the conversion of 2-phospho-Dglycerate into phosphoenol pyruvate, and is an essential enzyme in energy metabolism in nervous tissues.

NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury.

Image: shows the human embryonic kidney cells line 293 (Hek293), which express many neuronal proteins (1). The red channel shows staining with NSE, which recognizes all of these 293 cells. The green channels shows staining for another neuronal marker with mouse monoclonal to ubiquitin C-terminal hydrolase 1 (UCHL1) antibody. This neuronal gene is apparently activated in a cell density dependent fashion and at this stage only a few cells express this protein. However all cells that express NSE also express UCHL1.

More Links:

Neuron Specific Enolase (NSE)/ENO2-Antibody-Chicken

Neural/Glial Marker Antibodies

                                                     

Neurofilament or NF Markers

Stem Cell Research Reagents                               

Neurotrophins-Neural/Glial Expressed Proteins

Primary Neurons and Astrocytes-Primary human, rat and mouse neurons and astrocytes
 

Picture[2]

Image: Blot of rat spinal cord probed with rabbit antibody to NSE. The antibody stains a single sharp band corresponing to NSE at about 47kDa. Protocol on data-sheet.