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Type: Kit
 
Description/Data:
Magic Red Apoptosis Kit-CathepsinB

Image: Lipopolysaccharide-primed J774A.1 cells were incubated with or without DCA (100 μM, 24 h); then, the cells were stained with Lyso Tracker Green DND-26 (Invitrogen) or Magic RedTM Cathepsin B fluorogenic substrate z-Arg-Arg cresyl violet (Neuromics) for 1 h, followed by Hoechst staining for half an hour. Fluorogenic signals were captured by inverted fluorescence microscope (Leica). Front. Immunol., 28 November 2016 http://dx.doi.org/10.3389/fimmu.2016.00536

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Cathepsins are usually characterized as members of the lysosomal cysteine protease (active site) family and the cathepsin family name has been synomonous with lysosomal proteolytic enzymes.  In actuality, the cathepsin family also contains members of the serine protease (cathepsin A,G) and aspartic protease (cathepsin D,E) families as well. 

Image: Detection of intracellular cathepsin K activity in THP-1 cells using (z-LR)2-MR-Cathepsin fluorogenic substrate

These enzymes exist in their processed form as disulfide-linked heavy and light chain subunits with molecular weights ranging from 20-35 kDa.  Cathepsin C is the noted exception, existing as an oligomeric enzyme with a MW ~200 kDa.  Initially synthesized as inactive zymogens, they are post-translationally processed into their active configurations after passing through the endoplasmic reticulum and subsequent incorporation into the acidic environment of the lysosomes.

Protease activity can be detected within whole living cells using Neuromics’ Magic Red™ substrate-based assay kits.  Designated as the MR-Cathepsin product line, these kits allow researchers to quickly visualize intracellular cathepsin activity within their particular experimental cell line.

All Magic Red™ Real Time! Kits

FLISP™ Serine Protease Detection Kits

Measure chymotrypsin-like protease
activation in whole living cells.
 

FLIVO™ Polycaspase Live!, in vivo Apoptosis Kits-New

FLICA™ in vitro Caspase Kits
Fast!-Use Caspase kits to quantitate apoptosis via active caspases in whole, living cells. These kits do not use ELISA or any antibodies for detection
FLISP™ Serine Protease Detection Kits
Measure chymotrypsin-like protease
activation in whole living cells.
Magic Red™ Real Time! Kits
Measure apoptosis in whole living, intact cells - no lysis required
MitoPT™ Mitochondrial Permeability Transition Kit
Quantitate apoptosis and mitochondrial functionality -non-apoptotic cells with healthy mitochondria appear red,
apoptotic cells appear green.

Apoptosis Antibodies