Livin

Affinity Purified Rabbit Antibody
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Type: Goat IgG
 
Applications: WB
ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; WB=Western blotting; FC=Flow Cytometry; IP=Immunoprecipitation; E=ELISA; NB=Neutralization of Bioactivity; FACS; FM=Fluorescent Micsroscopy; ; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; TPE=Targeted Protein Expression; ; ; AC=Adherent Cell Assays; ; ; NAC=Non-adherent Cell Assays; ; ; BSC-CM5= Biacore Sensor Chip CM5; ;
Species Reactivity: H; R
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins
Format: Affinity Purified - liquid
 
Immunogen: Purified, E. coli-derived recombinant human Livin ?, also known as ML-IAP (amino acids 1 - 280), with a carboxyl-terminal six histidine tag (GenBank accession # AF311388).
 
Description/Data:
Picture

The protein encoded by this gene is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Two transcript variants encoding different isoforms have been found for this gene. The two isoforms have different antiapoptotic properties, with isoform alpha protecting cells from apoptosis induced by staurosporine and isoform b protecting cells from apoptosis induced by etoposide.

Image: Detection of Livin α and β. 0.5 ng of His tagged recombinant human Livin-α and human Livin-β , and lysates from 0.5 x 106 human SK-Mel-28 cells were resolved by SDS-PAGE, transferred to Immobilon membrane and immunoblotted with 0.5 μg/mL goat anti-Livin. A 60 second exposure to film is shown.

Related Reagents to Consider:

Survivin

Caspases and Cathepsins

All Apoptosis Reagents

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