FLICA® in vitro Caspase Kits

Easy to use, caspase inhibitor-based apoptosis detection kits that have been tested in primary neurons and many other cell types! 

Images: FLICA kits can tell you how your cells are responding to treatment.  In this experiment, 5 Jurkat cells were exposed to FAM-FLICA. 4 of them are apoptotic. They have active caspases and fluoresce bright green. (right).

Kit Features:
• Easy-Just add the reagent directly to the cell culture and incubate (see sidebar).
• Fast-The reactions start within 15 minutes of addition to the cells, however we recommend an incubation of 1-4 hours. 
• Accurate-There is no interference from pro-caspases or inactive forms of the enzyme. Quantitative Read with a fluorescence plate reader, microscope, or flow cytometer.
• Reliable-Only cells with active caspase enzymes fluoresce. The reagents may be incubated up to 72 hours without killing the cells
• Sensitive-Detects background levels of apoptosis in controls. Detect apoptosis earlier than Annexin V and TUNEL Caspases are released before the phospholipid turnover & DNA laddering.