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KF1720125 Tests$179.00Buy Now | Add to Cart
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Type: Kit
 
Description/Data:

Polycaspase and Caspase in vitro apoptosis kits make it easy to measure cell death and apoptosis in cultured neurons and many other cell types. Just add the reagent to the media, let it incubate 1- 4 hours, wash the cells and read with a fluorescence plate reader, microscope, or flow cytometer.  Apoptotic cells fluoresce red or green.

FLICA Caspase Detection Kits-FAQs

Picture

Images: Suspension cells were treated with an apoptosis-inducing agent or DMSO, a negative control, for 4 hours, washed twice, then incubated with ICT’s green caspase 1 inhibitor probe, FAM-YVAD-FMK, for 1 hour and examined under a fluorescence microscope. The top images reveal several experimental cells, all of which fluoresce green, therefore they have active caspase 1. The lower brightfield image at right reveals many control cells in the field of view, however the corresponding fluorescence image is dark (lower left); none of these cells have active caspase 1. Courtesy of Dr. Brian W. Lee, ICT.