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KF1720425 Tests$179.00Buy Now | Add to Cart
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Type: Kit
 
Description/Data:

Polycaspase and Caspase in vitro apoptosis kits make it easy to measure cell death and apoptosis in cultured neurons and many other cell types. Just add the reagent to the media, let it incubate 1- 4 hours, wash the cells and read with a fluorescence plate reader, microscope, or flow cytometer.  Apoptotic cells fluoresce red or green.

FLICA Caspase Detection Kits-FAQs

Picture

Data: Jurkat cells were treated with staurosporine, an apoptosis-inducing agent (bottom), or DMSO, a negative control (top), for 4 hours, then incubated with ICT’s green caspase 6 inhibitor probe, FAM-VEID-FMK, for 1 hour. Cells were washed twice and read on a flow cytometer. Treatment with staurosporine induced caspase 6 activity in 95.2% of the experimental cells (P3, bottom right), whereas the negative control treatment exhibited caspase 6 activity in only 5.9% of the cell population (P3, top right). This is a ratio of 16:1. Courtesy of Dr. Brian W. Lee, ICT.