hN2™ Human Neurons Discovery & HT Screening Kits
Stable, Potent and Well Characterized
hN2™ Kits can generate many neuronal phenotypes, including cells positive for the following: HB9 for motoneurons, ChAT for cholinergic neurons, DAT for dopaminergic neurons, GABA for GABAergic neurons, (Images), all cells relevant to studying various diseases as well as usable in assays for general toxicity not relevant to cellular phenotype. For The hN2™ Discover Kits, the cells have undergone two weeks of differentiation. The universal neural cell marker Tuj (beta tubulin III) indicates that >80% of the hN2™cells are neural. The hN2™cells possess electrically active membranes and the hN2™cells produce uniform responses in FLIPR glutamatergic pharmacology studies.
Images: Neural phenotypes derived from hN2 cell lines. (A) Phase contrast image of differentiated culture. (B) Network including post-mitotic motoneurons (HB9). (C) Cholinergic neuron. (D) Tuj-1 positive cells that are DAT-positive (dopamine transporter; closed arrow) and DAT-negative (open arrow). (E) Gabaergic neurons, inset illustrates GABA in axon, but not the dendrites (arrow). hN2 Cells-Electro Phys Data.
Customer Testimonial: "The cells grew very well, no problems at all. I will try to get some more images together over the next few days". Dr. Gerry Shaw, University of Florida
Note: hN2™ HTS Kit is an excellent choice for high-throughput (96- and 384-well) formatted assays.
Research use only
Markers for cellular phenotype and related disease
ChAT (Choline acetyltransferase) – synthetic enzyme
HB9 – transcription factor marker in motoneurons (cholinergic)
Dopamine transporter (DAT)
ALS, spinal injury, spinal muscular atrophy (SMA)
Parkinson’s disease, schizophrenia, depression, addictive disorders
Pain processing, conscious state (anesthesiology), alcohol research
|hN2™ Human Neurons Discovery Kit||HN60000||Primary Cells||H||Cell Assays||1,000,000 Cells||$795|
|hN2™ Human Neurons High Throughput Screening (HTS) Kit||HN60002||Primary Cells||H||Cell Assays||4,000,000 Cells||$1,395|
|hN2™ Human Neurons Culture Media||M60100||Cell Growth Media||H||500 ml & 2X5 ml||$250|