Protocol User's Guide for Culturing Motor Neuron on MEA(pdf - 679Kb)
ArunA Biomedical's/Neuromic's Mouse Motor Neurons on Axion Biosystem's MEA

Optimize ArunA Biomedical's/Neuromics GFP Labeled Mouse Motor Neurons  on Axion Biosystems' MEA!

This Protocol (User's Guide) will enable you to optimize growth of our mouse Hb9 ESC-derived motor neuron cultures on Axion’s MEA plates and dishes. Neurons cultured using this protocol should show spike activity detectable in AxIS software by Day 3. in vitro. The investigation of motor neuron electrophysiology has significance in diseases such as ALS and various palsies. Interactions and death of motor neurons are implicated in these disease states. The ArunA Biomedical/Neuromics mMNGFP+ Mouse Motor Neurons and Axion’s MEAs provide a useful platform for assay development. Figure: ArunA Biomedical's/ Neuromics' mMNGFP+ Mouse Motor Neurons Placement ArunA mMNGFP+ Mouse Motor Neurons (60,000) at day 6 in vitro in a 12-well MEA, 4x magnification. Notice the dotting method confines the majority of cells to the area indicated by the dotted line surrounding the grid of circular electrodes.

Axion Bioystem's/Aruns Biomedical's Motor Neurons/MEA Webinar Presentation.

NameCatalog #TypeSpeciesApplicationsSizePrice
Motor Neurons-GFP+ Quick Start KitmMN7205.QSPrimary NeuronsMCell Assays750,000$425
Motor Neurons-GFP+ HTS KitmMN7205-HTSPrimary NeuronsMCell Assays4 X 750,000$1,199
GDNF (Human, Mouse)PR27022-2ProteinH; M2 ug
10 ug
AB2™ Basal Neural MediumM60103Cell Growth MediaH; MCell Assays500 ml$79