Motor Neurons MEA
Protocol and Webinar
Optimize ArunA Biomedical's/Neuromics GFP Labeled Mouse Motor Neurons on Axion Biosystems' MEA!
This Protocol (User's Guide) will enable you to optimize growth of our mouse Hb9 ESC-derived motor neuron cultures on Axion’s MEA plates and dishes. Neurons cultured using this protocol should show spike activity detectable in AxIS software by Day 3. in vitro. The investigation of motor neuron electrophysiology has significance in diseases such as ALS and various palsies. Interactions and death of motor neurons are implicated in these disease states. The ArunA Biomedical/Neuromics mMNGFP+ Mouse Motor Neurons and Axion’s MEAs provide a useful platform for assay development. Figure: ArunA Biomedical's/ Neuromics' mMNGFP+ Mouse Motor Neurons Placement ArunA mMNGFP+ Mouse Motor Neurons (60,000) at day 6 in vitro in a 12-well MEA, 4x magnification. Notice the dotting method confines the majority of cells to the area indicated by the dotted line surrounding the grid of circular electrodes.
|Motor Neurons-GFP+ Quick Start Kit||mMN7205.QS||Primary Neurons||M||Cell Assays||750,000||$425|
|Motor Neurons-GFP+ HTS Kit||mMN7205-HTS||Primary Neurons||M||Cell Assays||4 X 750,000||$1,199|
|GDNF (Human, Mouse)||PR27022-2||Protein||H; M||2 ug|
|AB2™ Basal Neural Medium||M60103||Cell Growth Media||H; M||Cell Assays||500 ml||$79|