Catalog NumberSizePrice (USD)Shopping Cart
mMN7205-HTS4 X 750,000$1,199.00Buy Now | Add to Cart
 
Type: Primary Neurons
 
Applications: Cell Assays
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Species Reactivity: M
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Frozen
 
Description/Data:
Image of GFP+ Mouse Motor Neurons

Our new GFP+ Mouse Motor Neurons express > 25% eGFP expressing mouse motor neurons differentiated from an embryonic stem cell line (HBG3) expressing eGFP under control of the HB9 promoter. A specific motor neuron maker, HB9 allows for easy identification and tracking of motor neuron cells.

One vial of Motor Neurons should be thawed into two 35 mm dishes+500 ml Culturing Media!  Additional Supplies: We use TPP tissue culture dishes. Prior to thaw, the tissue culture dish will need to be precoated with Matrigel™ (BD Cat# 356234) using the following protocol. . We include 500 ml of the AB2™ Basal Neural Medium . Please note these supplements are also required:

  • Advanced DMEM/F12 (Life Technologies 12634-010) AB2™ Basal Neural Medium (Neuromics AB27011.3)-Optional if more than 500 ml is required.
    L-Glutamine (Life Technologies 25030- 081) 100 X 1 X 0.5 mL
  • Knockout Serum Replacement (Life Technolgies 10828010)
    Pen/Strep (Life Technologies 15070- 063) 100 X 1 X 0.5 mL
  • B- mercaptoethanol (Sigma M7522) 1 M (diluted in PBS -/- , stored @ -20 C) 0.1 mM 5 µL
  • GDNF (Neuromics PR27022) 100 µg/mL in H2O 10 ng/mL 5 µL Purchase options: 2 ug:=108 USD/ 10 ug= 205 USD
  • CTNF (Sigma C3710) 100 µg/mL in PBS w/ 0.1% BSA 10 ng/mL 5 µL

Images: Panel images of GFP+ mMN Mouse Motor Neurons at 2 days post thaw 20X

Cell Type:   Mouse motor neurons
Part Number:  7025
Species:  Mus musculus
Source:  HB9-GFP (HBG3) ESC
Morphology:  neural
Quantity: 750,000 cells per vial 
Shipping method:  dry ice
Storage:  liquid nitrogen
Growth Properties: adherent, non-proliferative
Medium:  AB2 Basal Neural Medium and supplements

Pathogen testing:  Cells are tested for sterility and mycoplasma contamination.

Viability testing:  Post-cryostorage viability was determined to be approximately 75%. 

Kit format: GFP+mMN™ Mouse Motor Neurons (Cat#mMN7025), 1 vials (~750,000 cells/vial post thaw)

Research use only 

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