|Catalog Number||Size||Price (USD)||Shopping Cart|
|mMN7205-HTS||4 X 750,000||$1,199.00||Buy Now | Add to Cart|
Our new GFP+ Mouse Motor Neurons express > 25% eGFP expressing mouse motor neurons differentiated from an embryonic stem cell line (HBG3) expressing eGFP under control of the HB9 promoter. A specific motor neuron maker, HB9 allows for easy identification and tracking of motor neuron cells.
One vial of Motor Neurons should be thawed into two 35 mm dishes+500 ml Culturing Media! Additional Supplies: We use TPP tissue culture dishes. Prior to thaw, the tissue culture dish will need to be precoated with Matrigel™ (BD Cat# 356234) using the following protocol. . We include 500 ml of the AB2™ Basal Neural Medium . Please note these supplements are also required:
- Advanced DMEM/F12 (Life Technologies 12634-010) AB2™ Basal Neural Medium (Neuromics AB27011.3)-Optional if more than 500 ml is required.
L-Glutamine (Life Technologies 25030- 081) 100 X 1 X 0.5 mL
- Knockout Serum Replacement (Life Technolgies 10828010)
Pen/Strep (Life Technologies 15070- 063) 100 X 1 X 0.5 mL
B- mercaptoethanol (Sigma M7522) 1 M (diluted in PBS -/- , stored @ -20 C) 0.1 mM 5 µL
GDNF (Neuromics PR27022) 100 µg/mL in H2O 10 ng/mL 5 µL Purchase options: 2 ug:=108 USD/ 10 ug= 205 USD
CTNF (Sigma C3710) 100 µg/mL in PBS w/ 0.1% BSA 10 ng/mL 5 µL
Images: Panel images of GFP+ mMN Mouse Motor Neurons at 2 days post thaw 20X
Cell Type: Mouse motor neurons
Part Number: 7025
Species: Mus musculus
Source: HB9-GFP (HBG3) ESC
Quantity: 750,000 cells per vial
Shipping method: dry ice
Storage: liquid nitrogen
Growth Properties: adherent, non-proliferative
Medium: AB2 Basal Neural Medium and supplements
Pathogen testing: Cells are tested for sterility and mycoplasma contamination.
Viability testing: Post-cryostorage viability was determined to be approximately 75%.
Kit format: GFP+mMN™ Mouse Motor Neurons (Cat#mMN7025), 1 vials (~750,000 cells/vial post thaw)
Research use only