Cortex

Primary Rat Cortical Cells are live neurons isolated from micro-surgically dissected regions of day 18 embryonic Sprague/Dawley rat brain. These cells are prepared fresh each week and shipped in a nutrient rich medium that keeps the cells alive for up to 14 days under refrigeration.

Customer Publications

Images: Pak1 colocalizes with muhtt aggregates. (A) Muhtt548-GFP and Pak1 were transfected into rat E18 striatal neurons. After 48 h, neurons were fixed and stained with anti-Pak1 antibody, then imaged with confocal microscopy. The outline of the neuron is shown in the merge picture. lease note that neurons expressing mutant huntingtin are frequently unhealthy, thus they do not show the normal neurite extensions seen in primary neurons expressing wild-type huntingtin or no transgene. Bar, 10 µm. (B) Muhtt548-GFP was transfected into rat E18 cortical neurons. After 72 h, neurons were fixed and stained with anti-Pak1 antibody, then imaged with confocal microscopy. Note Pak1 staining decorating endogenous Pak1. The outline of the neuron is shown in the merge picture. Bar, 10 µm. (C) HD brain (Grade III) slices were stained with anti-htt (EM48) and anti-Pak1, anti-htt (EM48) only or anti-Pak1 only or no primary antibodies using PicTure-Double staining kit. The secondary antibodies, anti-mouse IgG-HRP and anti-rabbit IgG-AP were applied to all brain slices. The control brain sample slices were stained with anti-htt (EM48) and anti-Pak1 antibody. Inclusions decorated with Pak1 or/and htt were seen in HD samples, (example shown), but not in HD slice stained with no primary antibodies, or control slice stained with anti-htt and anti-Pak1. Bar, 10 µm. (D) HD brain (Grade III) and control brain samples were stained with anti-Pak1 antibody using Vectastain Avidin kit. Inclusions decorated with Pak1 was seen in all three HD samples (example shown), but not in controls (data not shown). Bar, 10 µm. . Human Molecular Genetics 2008 17(6):895-905; doi:10.1093/hmg/ddm362.

Companion Products:

NbActiv4^TM-neuron culture medium is an improved version of Neurobasaltm / B27tm.  NbActiv4 grows neurons with twice as many synapses and up to 8x higher spike rates than Neurobasal/B27.

Poly-D-Lysine Coated Coverslips-2 Glass coverslips, (1.76cm2) coated with poly-D-lysine, sterile, in PBS, shelf-life 1 week, per pair.

Please note we also provide Adult Neurons in Qtys of 4 (or more) for $995.  To order call brett @ 866-350-1500 or e-mail: brett@neuromics.com.