HALO® in vitro Cell Based Assays

Designed for Lympho-Hematopoietic Stem and Progenitor Cell
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HALO® assays are non -subjective, instrument based and quantitatve. They give you the ability to measure intracellular ATP (iATP) to determine viability, functionality, proliferation, cytoxicity and cell number.  All of which are proportional to iATP. Results are fast, easy to determine and cost effective.

HALO® Assays for Human Lympho-Hematopoietic Stem and Progenitor Cell Research.

Applications:
  • For all human lympho-hematopoietic research applications.
  • Detect and measure proliferation of human stem and progenitor cells.
  • Measure and compare proliferation status and/or potential between human tissues and cell populations.
  • High-throughput Hemotoxicity screening.
  • Experimental transplantation models.
  • Effects of growth factors and/or cytokines. In vivo to in vitro assays. Gene targeting assays.

Human Cell Populations Detected using HALO®-96

Stem Cells
  • HPP-SP 1: IL-3, IL-6, SCF, TPO, Flt3-L HPP-SP 2:
  •  EPO, GM-CSF, IL-2, IL-3, Il-6, IL-7, SCF, TPO, Flt3-L CFC-GEMM 1:
  • EPO, GM-CSF, IL-3, IL-6, SCF, TPO, Flt3-L
  • CFC-GEMM 2: EPO, GM-CSF, IL-3, IL-6, SCF, TPO
  • CFC-GEM 1: EPO, GM-CSF, IL-3, IL-6, SCF
  • CFC-GEM 2: EPO, GM-CSF, IL-3, SCF
  • CFC-GEM 3: EPO, GM-CSF, G-CSF, IL-3, SCF
Progenitor Cells
  • BFU-E 1: EPO, IL-3, SCF
  • BFU-E 2: EPO
  • GM-CFC 1: GM-CSF, IL-3, SCF
  •  GM-CFC 2: GM-CSF, G-CSF, IL-3, SCF GM-CFC 3: GM-CSF
  • Mk-CFC 1:
  • TPO, IL-3, SCF
  • Mk-CFC 2: TPO
Lymphopoietic cells
  • T-CFC: IL-2
  • B-CFC: IL-7

Note: No growth factors: Add growth factors/cytokines of choice.

Biolumonics Basic Procedures(pdf - 323Kb)
Fast and easy to learn
HALO® 96 HuSPCA Cell Based Assay Kits
Designed for Human Lympho-Hematopoietic Stem and Progenitor Cell Assays
Selecting HALO™ Assays(pdf - 1.7M)
Which is right for you?
HALO®-96 Tox and HALO®-384 HT
Designed for toxicity studies in Human/Animal Lympho-Hematopoietic Stem and Progenitor Cell Assays

Designed for Lympho-Hematopoietic Stem and Progenitor Cell

  
HALO® 96 HuSPCA Cell Based Assay Kits
Designed for Human Lympho-Hematopoietic Stem and Progenitor Cell Assays
HALO®-96 Tox and HALO®-384 HT
Designed for toxicity studies in Human/Animal Lympho-Hematopoietic Stem and Progenitor Cell Assays