Human Mesenchymal Stem Cells-Green Fluorescent Nanoparticle-labeled

Cord Blood-Derived Cyro-preserved Cells
hMSCS-Protocol for culturing, passaging and Cryo-preservation(pdf - 130Kb)
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SC00A4NP500,000$450.00   $550.00Buy Now | Add to Cart
 
Type: Stem Cells
 
Applications: MSC Cell Assays
E=ELISA; FC=Flow Cytometry; ICC=Immunocytochemistry; IF=Immunofluorescence; IP=Immunoprecipitation; IHC=Immunohistochemistry; SE=Sandwich ELISA; WB=Western blotting; NB=Neutralization of Bioactivity; FACS; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; BSM=Biosactive Small Molecule or Peptide; HPLC=High Performance Liquid Chromatography; TPE=Targeted Protein Expression; AC=Adherent Cell Assays; NAC=Non-adherent Cell Assays; CDM=Cell Differentiation Media; BSC-CM5= Biacore Sensor Chip CM5; FM=Fluorescent Micsroscopy; ; ; ; ; ; ; ; ; ;
Species Reactivity: H
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All
Format: Frozen
 
Description/Data:
Picture

Intro Special Save 100 USD!

These cells are generated by chemical transfection of CdSe/ZnS nanoparticles, 100 nm in diameter and show a strong fluorescent signal (Excitation maximum= 515 nm; Emission maximum= 520-550 nm) detectable by standard FITC filters.

Image: FITC analysis of cells during fourth passage following transfection. Image obtaining using FITC filter on Olympus CKX41 microscope at 200 X equipped with a Retiga 2000 digital camera and Q-Capture software program. Scale bar is 25 micrometers. Fluorescent nanoparticles are less prevalent within the cytoplasm than earlier passages. Technical Information & Data.

They retain GFNP labeling through 3+ passages and can be differentiation into chondrogenic, adipogenic or osteogenic lineages. Excellent for regeneration, toxicity and related studies.

This product requires shipment on dry ice and immediate transfer to liquid nitrogen storage or culture. Please note there is a 33 USD dry ice charge in addition to Fedex next day shipping.

Optimizing your hMSC Cultures:
1)     Grow cells in 1 to 5% O2, 5% CO2, with the balance of the gas content as N2..
2)      Use of our MSCGroTM media is highly recommended.
3)     Subtrate- We recommend Greiner Bio-one TC-treated cell culture flasks or equivalent.

More Links:

 Stem Cell Research Antibodies  Stem Cell Research Proteins  Stem Cell Research Reagents
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Image: Osteogenic differentiation of GF nanoparticle-labeled MSCs. Image obtaining using phase contrast Olympus CKX41 microscope at 100 X equipped with a Retiga 2000 digital camera and Q-Capture Pro software program. Scale bar is 25 micrometers. Note the presence of mineral deposits indicating differentiation into osteoblasts. Chondrogenic and adipogenic differentiation was also apparent.