Human Melanoma Cancer Associate Fibroblasts are isolated from malignant melanoma and cryopreserved at a low passage. These cells expand in VitroPlus III Low serum, complete medium (Cat# PC00B1) and have high passage capabilities.
Image: Melanoma cancer-associated fibroblasts (Cat# CAF09) were plated at 5,000/cm² in a Greiner BioOne T75 flask and maintained in MSC-GRO low serum, complete medium (Cat# SC00B1) in a reduced O₂ environment (1%O₂, 5%CO₂, 90%N₂) at 37°C in a humidified chamber. The CAFs were harvested and counted on a Beckman-Coulter Z2 particle counter (range 10-30uM). Doubling time of 30hrs was calculated using ln(2*dT)/ln(Cf/Ci), where dT is the time, in hours, from inoculation to detachment; Ci is the initial number of cells plated and Cf is the final number of cells recovered from subculture.