Astroglial-Neuron Co-Culture Data

The Balance of Astroglia-Neurons in Your Cultures Matter-in vivo Like Ratios Produces More Accurate Data (see figure). 

Our Strategic Partner, ArunA Biomedical, has made available to us the first of  their kind human astrocyte-glial or astrocyte-glial-neuron co-culturing kits to meet your exacting specifications. Whether you require a pure astro-glial or mixed culture, we are proud to be the first offering you the right solution. Options:

  1. hAstroPro™ Human Astrocyte Cells are derived from human pluripotent stem cell line h9 (NIH registry number 0062) and are the first highly characterized hESC derived CNS support cell type commercially available. hAstroPro™ cells are derived using a chemically defined method that enables shortened differentiation and scaled uniform production.
  2. hAstroPro™+NeuroNet™ Pure Human Neurons are a highly pure population of hPSC derived neurons developed with versatility in mind. As a standalone model for studies of cellular and molecular neurobiology, drug discovery and neurotoxicity, NeuroNet™ Human Neurons enable rapid response in a relevant in vitro culture model system and have been strategically designed for co-planer cellular model studies with

Contact us for a quote today: pshuster@neuromics.com or direct phone 612-801-1007. Pricing 1500-3500 USD depending on culturing and protocol requirements.

 

hAstroPro Human Astrocyte Kit

Cell Type:  Astrocytes
Part Number:  hAP7027
Species:  human
Source:  derived from h9 hESC (NIH registry number 0062)
Morphology:  glial
Quantity:  ~1x106cells per vial
Shipping method:  dry ice
Storage:  liquid nitrogen
Growth Properties:  adherent
Medium:  AB2™ Basal Neural Medium with ANS™ Neural Medium Supplement plus additional unsupplied reagents.
Purity as determinded by qPCR: S100β, CD44, BDNF, GFAP
.

DNA fingerprint:  Results are consistent with the presence of a single cell line with no evidence of culture cross contamination. The loci match the DNA fingerprint pattern for the H9 (NIH designation, WA09) hESC line as published in http://stemcells.nih.gov/research/nihresearch/scunit/.

NeuroNet Pure Human Neurons

Quantity:  ~1x106 cells per vial
Shipping method:  dry ice
Storage:  liquid nitrogen
Growth Properties:  adherent, non-proliferative
Medium:  AB2™ Basal Neural Medium with ANS™ Neural Medium Supplement plus LIF (10 mg/mL)
Phenotyping:  Cells are >90% β III tubulin positive and >60% MAP2 positive.

Pathogen testing:   This lot was derived from the H9 hESC line that has been tested for Hepatitis B, Hepatitis C, HIV-1, HIV-2, HTLV-I/II, HSV1, HSV2, EBV, and CMV.  The H9 cell line has been tested and shown to be negative (performed by GIVF Laboratories).  Cells are tested for sterility and mycoplasma contamination.