Catalog NumberSizePrice (USD)Shopping Cart
3D45002-66 wells$1,780.00Buy Now | Add to Cart
3D45002-1212 well$2,600.00Buy Now | Add to Cart
3D45002-2424 well$3,625.00Buy Now | Add to Cart
NMBBB01500 ml - Media$295.00Buy Now | Add to Cart
 
Type: 3D Cell/Tissue Models
 
Applications: In vivo Like Assays
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Description/Data:
3-D Blood Brain Barrier Model

Our model mimic transport properties of the Blood Brain Barrier (BBB) due to the formation of tight junctions, higher expression of specific carriers, or great cell viability. We developed a 3D in vitro model of the BBB by culturing brain endothelial cells with pericytes and astrocytes layered in an insert. This model improve endothelial cell polarization and enhance the formation of tight junctions, provide better endothelial cell-to-cell contact that is important for barrier development, and prevent the dilution of secreted neurotrophic factors, and these conditions collectively led to the development of an in vitro model that can truly mimic the BBB.

Models are made to order. Please allow for 3-5 weeks for completion. 

Models come with media. Additional media can be ordered (catalog # NMBBB01). 

Detailed Images of BBB Model Capabilities

Images: Expressions of BBB transporters in our 3D Human BBB Model

The 3D Human BBB model can be use, but not limited to:
  • Drug BBB permeability assay
  • Research on BBB physiology
  • Cell-cell interactions
  • Transport pathway modulations
  • Research on BBB toxicology
  • Brain endothelial toxicity assays
  • Research on BBB pathology
  • Disease modeling                                                                    
  • Transport and permeability studies from ions to macromolecules: effect of physiological or pathogenetic factors
  • Paracellular barrier and cell polarity studies: TJ protein expression, distribution, polarized distribution of transport proteins, receptors, enzymes etc
  • Studies on endo- and transcytosis, receptor-ligand interactions
  • Drug transport, drug effect on permeability, localization of receptors, polarity of drug responses
  • Co-culture studies: cell-cell and cell-matrix interactions
  • Microbial pathogenesis: virus, bacteria, parasite attachment, invasion and penetration