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Type: 3D Cell/Tissue Models
 
Applications: In vivo Like Assays
E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays
Description/Data:
Image HUVEC ModelImage of 3-D Angiogenesis Assay

Angiogenesis is a multistep process whereby new blood vessels develop from preexisting vasculature. Angiogenesis plays a key role in numerous physiological and pathological processes and understanding the mechanism of angiogenesis will therefore provide new approaches to the treatment of a wide range of pathologies. Angiogenesis is a complex process in which the following events are believed to play a critical role:   

  • Proteolytic degradation of the extracellular matrix
  • Directed migration of endothelial cells
  • Proliferation of endothelial cells  
  • Deposition of new extracellular matrix
  • Formation of tubules and anastomosis of the newly formed vessels 
Image: HUVECs growing in inserts         

                                              

Our 3D Human HUVECs Angiogenesis model is constructed using GFP-Tagged human umbilical vein endothelial cells (HUVECs) are co-cultured with RFP-Tagged supporting cells. GFP positive capillary like tubule formation can be monitored in real time under fluorescence microscope throughout the whole process of the experiment.

The 3D model is designed that the testing materials, i.e. compounds, conditioned media, or tissue explants, can be added into the system at any time, ranging from the onset of vasculogenesis to advanced angiogenesis. The resulting effect on tubule formation (tubular length, number of branches, etc.) can be monitored throughout the whole process under inverted fluorescence microscope.

Image: Left: 4x overlay image of HUVECs used in an endothelial  cell capillary tube formation assay. The cells were stained with calcein.Imaging was performed in the brightfield and GFP channels. Right: Co-culture system

Image of 3-D Angiogenesis Assay