Short Protocol
Human Cell Isolation with Fab Streptamer® Microbeads
Example of CD8+ target cell enrichment from human PBMCs

Streptamer® Driven T-Cell Isolation-Selection

Streptamers are used for efficient isolation of cells. T-Cells are separated from other cells by positive selection in a strong permanent magnetic field.

Figure: Example of CD8+ target cell enrichment from human PBMCs. Isolation of CD8+ cells from human PBMCs using recombinant CD8 Fab-Strep multimerized on Strep-Tactin® Magnetic Microbeads. live (PInegative) cells are shown

In a second step, the isolated cells are liberated from all labeling reagents by the addition of D-biotin (vitamin H) and washing in order to obtain a functional, completely label-free target cell preparation. The Fab-Strep ligands are engineered to have week affinity to the cognate cell surface receptor and the monomeric ligand therefore can be rapidly removed from its receptor by simple washing steps.

Fab Streptamer T-Cell Isolation Process

 

 

 

 

 

 

Figure: Receptor specific target cells are first labeled with fluorescent Fab Streptamer® (complexes of Fab-Streps and Strep-Tactin® PE or APC). In a second step, the isolated cells are liberated from all labeling reagents by addition of D-biotin (vitamin H) and washing in order to obtain a functional, completely label-free receptor specific cell preparation.

NameCatalog #TypeSpeciesApplicationsSizePrice
StrepMan Magnet for 15ml and 50ml Tubes6-5650-065Cell Isolation-SeparationHCell Assays1 Magnet$400
CD3 Fab Streptamer Isolation Kit MB6-8000-201Cell Isolation-SeparationHCell Assays1 Kit$699
CD4 Fab Streptamer Isolation Kit MB6-8000-206Cell Isolation-SeparationHCell Assays1 Kit$699
CD8 Fab Streptamer Isolation Kit MB6-8000-203Cell Isolation-SeparationHCell Assays1 Kit$699
CD25 Fab Streptamer Isolation Kit MB6-8000-207Cell Isolation-SeparationHCell Assays1 Kit$699
Strep-Tactin® PE for Fab Streptamers6-5001-010Fluoro-StainHFACSFor 1 x 10^8 cells$295
Strep-Tactin® APC for Fab Streptamers6-5011-010Fluoro-StainHFACSFor 1 x 10^8 cells$295