Sleeping Beauty Gene Integration Process

Gene Integration at Fractions of the Cost vs Other Technologies

We are pleased to announce the addition of Sleeping Beauty TransposonTM Systems courtesy of our Friends at B-MoGen Biotechnologies. This robust system represents a fast, potent and cost effective method for integrating reporter genes into the stem/primary cells or cell lines of your choosing.

Figure: Sleeping Beauty TransposonTM Systems Gene Integration Process. (A) A depiction of Sleeping Beauty transposon plasmid and Sleeping Beauty transposase enzyme active in cellular nuclei. (B) Transposase enzyme binds to Sleeping Beauty-specific IR/DR sites. (C) Transposase enzyme excises transposon sequence from transposon vector. (D) Transposase enzyme stably integrates transposon sequence at TA site in host cell genome.

Features Include:
  • Simple-"Cut and Paste" Integration and Editing. Check out our Sleeping Beauty Transposon System Users Manual
  • Potent-100% Stable with No Off target effects with the safest transposon insertion profile.
  • Fast- Transfect with our transposase + Sleeping Beauty TransposonTM Reporter Series Vectors.
  • Cost Effective- Starting at $329
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Neuromics' Sleeping Beauty vectors come with different promoters and reporters, ready for co-transfection with our Sleeping Beauty transposase expression vectors.

Our SBT Reporter Series vectors come ready for easy reporter gene transfer and stable reporter gene expression in your target cells. Our MCS (Multiple Cloning Site) SBT series vectors are an affordable way to generate your own custom Sleeping Beauty transposon vectors. Clone and ligate your desired gene into an MCS ready SBT vector to put your gene of interest into your cells.

Cytomegalovirus Promoter

Cytomegalovirus Promoter boasts high levels of gene expression in most mammalian cells, especially in neurons and embryonic stem cells.

CMV-EGFP
Sleeping Beauty Transposon
CMV-RFP
Sleeping Beauty Transposon
CMV-MCS-EGFP
Sleeping Beauty Transposon
CMV-MCS-Puro
Sleeping Beauty Transposon
CMV-MCS-Neo
Sleeping Beauty Transposon

EF-1 Alpha Promoter

The EF-1 Alpha promoter provides a stable, high-level of expression in most all mammalian cells. A great promoter to create brightly fluorescing cells and robust drug resistant cell lines.

EF1a-EGFP
Sleeping Beauty Transposon
EF1a-RFP
Sleeping Beauty Transposon
EF1a-MCS-EGFP
Sleeping Beauty Transposon
EF1a-MCS-Puro
Sleeping Beauty Transposon
EF1a-MCS-Neo
Sleeping Beauty Transposon

Phosphoglycerate Kinase Promoter

Phosphoglycerate Kinase promoter controls a steady level of constitutive gene expression across a wide variety of stem cells, neurons, and epithelial cells.

PGK-EGFP
Sleeping Beauty Transposon
PGK-RFP
Sleeping Beauty Transposon
PGK-MCS-Puro
Sleeping Beauty Transposon
PGK-MCS-Neo
Sleeping Beauty Transposon

EFS Promoter

The elongation factor 1α short, EFS, promoter is the miniaturized version of EF1α promoter. This makes it an ideal promoter when keeping your plasmids small is necessary for viable transfection into difficult cells

EFS-EGFP
Sleeping Beauty Transposon
EFS-RFP
Sleeping Beauty Transposon
EFS-MCS-Puro
Sleeping Beauty Transposon
EFS-MCS-Neo
Sleeping Beauty Transposon
EFS-MCS-EGFP
Sleeping Beauty Transposon

Ubiquitin C Promoter

The Ubiquitin C promoter is a staple choice for generating transgenic cell lines with stable consistent expression across a wide variety of mammalian species.

UBC-EGFP
Sleeping Beauty Transposon
UBC-RFP
Sleeping Beauty Transposon
UBC-MCS-Puro
Sleeping Beauty Transposon
UBC-MCS-Neo
Sleeping Beauty Transposon
UBC-MCS-EGFP
Sleeping Beauty Transposon

SBA vectors are Sleeping Beauty Transposase Expression Vectors that are ready to be co-transfected with our transposons vectors to begin generating transgenic cells.

CMV-SB11
Sleeping Beauty Transposase Expression Vector