i-Fect

i-Fect^TM is a novel cationic lipid formulation specifically designed for efficient delivery of siRNAs (small interfering RNAs) into a wide variety of cell types.  Customers have been using i-Fect for both in vitro and in vivo applications.

Kit Contents

• Hydrated i-Fect ™ Lipid
• siRNA Diluent

Figure:  siRNA-mediated suppression of target gene expression in Schwann cells. A, Detergent extracts of siNeg- or siGly1-transfected Schwann cells were digested with heparitinase and subjected to immunoblot analysis with anti-glypican-1 antibodies (top); aliquots of undigested extracts were immunoblotted with anti-actin antibodies (bottom) to verify equal sample loading. B, Cell surface expression of glypican-1 was assessed by immunofluorescent staining of transfected cells 48 h after transfection using anti-glypican-1 antibodies (green) and DAPI (4',6'-diamidino-2-phenylindole) to stain nuclei (red). C, Schwann cells were transfected with siNeg or si alpha4(V), and conditioned media and cell lysates were harvested 48 h later (left) or at the indicated times after transfection (right); aliquots of medium (top) or cell lysates (bottom) were subjected to immunoblot analysis and stained with anti-alpha4(V) collagen (top) or anti-actin (bottom) antibodies.

Modulating Sensory Systems Using RNAi. Josephine Lai, PhD, Miaw-Chyi Luo, PhD, Nicole Schechter, BS, Dongqin Zhang, MD, and Frank Porreca, PhD. © 2007 Lai. Department of Pharmacology.University of Arizona Health Sciences Center. Tucson, Arizona. Methods for Delivering RNAi in vivo using i-Fect

New publication-epidural delivery of siRNA 

Protocol

For High Throughput in vitro applications use: i-Fect ™ Hi Put 96-New

Other Reagents to Consider:

• MATra Magnetically Driven Transfection Techique
• siRNA-intact-protection from RNase activity
• pn-Fect
• n-Fect
• Penetratin 1