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Human Neuron specific enolase (NSE) (EC 184.108.40.206) is a 47,138 dalton protein (434 amino acids) expressed in neurons of the peripheral nervous system (PNS) and central nervous system (CNS). NSE catalyzes the conversion of 2-phospho-Dglycerate into phosphoenol pyruvate, and is an essential enzyme in energy metabolism in nervous tissues.
NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury.
Image: shows the human embryonic kidney cells line 293 (Hek293), which express many neuronal proteins (1). The red channel shows staining with NSE, which recognizes all of these 293 cells. The green channels shows staining for another neuronal marker with mouse monoclonal to ubiquitin C-terminal hydrolase 1 (UCHL1) antibody. This neuronal gene is apparently activated in a cell density dependent fashion and at this stage only a few cells express this protein. However all cells that express NSE also express UCHL1.
Image: Blot of rat spinal cord probed with rabbit antibody to NSE. The antibody stains a single sharp band corresponing to NSE at about 47kDa. Protocol on data-sheet.